A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis
A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis
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DOI：
10.1128/jb.182.8.2307-2310.2000
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发表时间：
2000-04-01
影响因子：
3.2
通讯作者：
Tsuchiya, T
中科院分区：
文献类型：
--
作者：
Masaoka, Y;Ueno, Y;Tsuchiya, T
从枯草芽孢杆菌ATCC 9372的染色体DNA中克隆了与乙锭抗性有关的基因（ebrAB）。重组质粒不仅在大肠杆菌中，而且在B中对溴化乙锭、吖啶黄、派洛宁Y和番红O产生了增强的抗性。枯草杆菌。它还引起E. EbrA和EbrB与多药外排泵的小多药耐药（small multidrug resistance，SMR）家族成员具有较高的序列相似性。ebrA和ebrB都不足以产生耐药性，但引入携带在不同质粒上的两种基因可产生耐药性。因此，EbrA和EbrB似乎都是多药外排泵活性所必需的。在已知的SMR家族成员中，只有一个基因产生药物外排。因此，EbrAB是一种新型的SMR家族多药外排泵，具有两个组件。
Genes (ebrAB) responsible for ethidium resistance were cloned from chromosomal DNA of Bacillus subtilis ATCC 9372, The recombinant plasmid produced elevated resistance against ethidium bromide, acriflavine, pyronine Y, and safranin O not only in Escherichia coli but also in B. subtilis. It also caused an elevated energy-dependent efflux of ethidiurn in E. coli, EbrA and EbrB showed high sequence similarity with members of the small multidrug resistance (SMR) family of multidrug efflux pumps. Neither ebrA nor ebrB was sufficient for resistance, but introduction of the two genes carried on different plasmids conferred drug resistance. Thus, both EbrA and EbrB appear to be necessary for activity of the multidrug efflux pump. In known members of the SMR family, only one gene produces drug efflux. Thus, EbrAB is a novel SMR family multidrug efflux pump with two components.