Transscleral iontophoresis of cefazolin, ticarcillin, and gentamicin in the rabbit.
Transscleral iontophoresis of cefazolin, ticarcillin, and gentamicin in the rabbit.
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DOI：
10.1016/s0161-6420(86)33780-1
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发表时间：
1986
期刊：
影响因子：
13.7
通讯作者：
M. Barza;C. Peckman;J. Baum
中科院分区：
文献类型：
--
作者：
M. Barza;C. Peckman;J. Baum
角膜离子导入会在有晶状体眼的眼前段产生高浓度的药物，但不会在有晶状体眼的玻璃体房水中产生高浓度的药物，这可能是因为晶状体-虹膜屏障阻碍了药物的运输。为了在有晶状体兔眼的玻璃体体液中产生高水平的常用抗生素，我们使用了两种改良法：(1)将设备放置在平坦部上方，绕过晶状体-虹膜屏障；(2)保持液柱的接触面积很小(≤直径1 mm)，以提供高电流密度。在正常兔眼内，施加2 MAMP 10分钟，可获得94-207微克/毫升的头孢唑林、替卡西林和庆大霉素的平均玻璃体浓度。药物渗透与电流强度(0.1-2 mAp)和离子导入时间(1-10分钟)相关，而与药液浓度无关。经巩膜离子导入是一种有前景的非侵入性技术，可以获得高水平的带电溶质。
Corneal iontophoresis produces high concentrations of drugs in the anterior segment but not in the vitreous humor of the phakic eye, presumably because transport is impeded by the lens-iris barrier. We have used two modifications to produce high levels of commonly used antibiotics in the vitreous humor of the phakic rabbit eye: (1) the device is placed over the pars plana, circumventing the lens-iris barrier; (2) the contact area of the column of fluid is kept small (≤ 1 mm diameter) so as to provide a high current density. Applying 2 mAmp for 10 minutes, we were able to achieve mean vitreal concentrations of cefazolin, ticarcillin, and gentamicin of 94–207 µg/ml in the normal rabbit eye. Drug penetration showed a correlation with strength of current (0.1–2 mAmp) and with duration of iontophoresis (1–10 minutes) but not with concentration of the drug solution. Transscleral iontophoresis is a promising noninvasive technique to achieve high vitreal levels of charged solutes.