Conformational preferences and binding to neurophysins of oxytocin analogs with sarcosine or N-methylalanine in position 7.
Conformational preferences and binding to neurophysins of oxytocin analogs with sarcosine or N-methylalanine in position 7.
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DOI：
10.1111/j.1399-3011.1985.tb02188.x
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Z. Grzonka;P. K. Mishra;A. Bothner-by
中科院分区：
文献类型：
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作者：
Z. Grzonka;P. K. Mishra;A. Bothner-by
600兆赫质子核磁共振谱。本文报道了（肌氨酰7）-催产素和（N-甲基丙氨酰7）-催产素在2H_2O溶液中的光谱，并对其进行了归属。在每种情况下，光谱表明存在两种缓慢相互转化的构象异构体，它们是残基6和7之间的肽键的顺反异构体。在这两种情况下，反式异构体在能量上都是有利的。当neurophysin被添加到溶液中的（N-甲基丙氨酰7）催产素或（sacrosyl 7）-催产素在pH 3.0，次要构象的比例保持不变，表明顺式和反式构象同样紧密结合的蛋白质。
The 600 MHz proton n.m.r. spectra of (sarcosyl7)-oxytocin and (N-methylalanyl7) oxytocin in 2H2O solution have been recorded and completely assigned. In each case the spectrum indicates the presence of two slowly interconverting conformers, which are the cis-trans isomers about the peptide bond between residues six and seven. The trans isomer is energetically favored in both cases. When neurophysin is added to a solution of (N-methylalanyl7) oxytocin or (sacrosyl7)-oxytocin at pH 3.0, the proportion of minor conformer remains constant, indicating that the cis and trans conformers are equally tightly bound to the protein.