Galectin-7 as a potential predictive marker of chemo- and/or radio-therapy resistance in oral squamous cell carcinoma.
Galectin-7 as a potential predictive marker of chemo- and/or radio-therapy resistance in oral squamous cell carcinoma.
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DOI：
10.1002/cam4.195
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发表时间：
2014-04
期刊：
影响因子：
4
通讯作者：
Omura, Ken
中科院分区：
文献类型：
--
作者：
Matsukawa, Sho;Morita, Kei-ichi;Negishi, Ayako;Harada, Hiroyuki;Nakajima, Yusuke;Shimamoto, Hiroaki;Tomioka, Hirofumi;Tanaka, Kae;Ono, Masaya;Yamada, Tesshi;Omura, Ken
关键词：
晚期口腔鳞状细胞癌（OSCC）的治疗需要多模式方法的整合。本研究的目的是确定肿瘤敏感性的预测因素，术前放疗/化疗的口腔鳞癌，以使肿瘤学家确定最佳的治疗策略，而没有相关的不良反应。在这里，福尔马林固定石蜡包埋（FFPE）组织样本的蛋白质表达谱从18个OSCC患者，称为学习的情况下，谁接受术前化疗和/或放疗后手术进行了分析，定量蛋白质组学和验证免疫组化在68个测试的情况下，以及在18个学习的情况下。我们确定半乳糖凝集素-7作为化疗和/或放疗耐药的潜在预测标志物，在68例测试病例中，半乳糖凝集素-7预测评分（G7 PS）预测这种耐药的灵敏度和特异性分别为96.0%和39.5%。G7 PS预测耐药患者的5年累积疾病特异性生存率为75.2%，敏感预测患者的5年累积疾病特异性生存率为100%。在体外过表达半乳糖凝集素-7显着降低OSCC细胞系的细胞活力。因此，我们的研究结果表明，半乳糖凝集素-7是一个潜在的预测标记物的化疗和/或放疗耐药的口腔鳞癌患者。鉴定来自敏感或耐药患者的OSSC样品中差异表达的蛋白质。通过LC-MS处理样品并用2DICAL分析。
Treatment of advanced oral squamous cell carcinoma (OSCC) requires the integration of multimodal approaches. The aim of this study was to identify predictors of tumor sensitivity to preoperative radiotherapy/chemotherapy for OSCC in order to allow oncologists to determine optimum therapeutic strategies without the associated adverse effects. Here, the protein expression profiles of formalin-fixed paraffin-embedded (FFPE) tissue samples from 18 OSCC patients, termed learning cases, who received preoperative chemotherapy and/or radiotherapy followed by surgery were analyzed by quantitative proteomics and validated by immunohistochemistry in 68 test cases as well as in the 18 learning cases. We identified galectin-7 as a potential predictive marker of chemotherapy and/or radiotherapy resistance, and the sensitivity and specificity of the galectin-7 prediction score (G7PS) in predicting this resistance was of 96.0% and 39.5%, respectively, in the 68 test cases. The cumulative 5-year disease-specific survival rate was 75.2% in patients with resistant prediction using G7PS and 100% in patients with sensitive prediction. In vitro overexpression of galectin-7 significantly decreased cell viability in OSCC cell line. Therefore, our findings suggest that galectin-7 is a potential predictive marker of chemotherapy and/or radiotherapy resistance in patients with OSCC. Identification of proteins differentially expressed in OSSC samples from patients sensitive or resistant. The samples were processed by LC-MS and analyzed with 2DICAL.
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