KINETIC-PROPERTIES OF THE BLUE-LIGHT RESPONSE OF STOMATA
KINETIC-PROPERTIES OF THE BLUE-LIGHT RESPONSE OF STOMATA
复制标题
DOI：
10.1073/pnas.82.23.8019
复制
发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
ZEIGER, E
中科院分区：
文献类型：
--
作者：
IINO, M;OGAWA, T;ZEIGER, E
利用气体交换技术研究了鸭跖草气孔对蓝光的响应。通过使用高通量率短脉冲离开。在高通量红光背景下发出蓝光脉冲，使光合作用维持在近饱和状态，气孔导度维持在稳定状态。1-100秒的蓝光脉冲使气孔导度增加，15 min后达到峰值，50-60 min后恢复到初始的稳态水平。在同一叶片中可重复诱导该反应。红色背景上的红光脉冲没有引起任何可比的反应。在脉冲施加后，通过积分电导率而量化的气孔响应随着脉冲持续时间（t1/2）的增加而接近饱和。9秒，250 μ mol·cm·dot。m-2.cntdot.秒-1的蓝光）。在饱和脉冲之后，对第二脉冲的敏感性缓慢恢复。从由两个脉冲引起的电导增加量化的该恢复响应以约t1/2接近饱和。这些结果用于测试模型，其中感觉转导过程中的分子组分被认为以两种可相互转化的形式A和B存在。如果B是诱导气孔开放的生理活性形式，则A是非活性形式。A到B的转化是光诱导反应，而B到A的转化是热反应。这些反应的速率常数由单脉冲和双脉冲实验（在250 μ mol·cm-1的注量率下）估算。m-2.cntdot. sec-1，k1 = 0.075 sec-1;热速率常数kd = 0.0014 sec-1），允许计算连续照射下B的稳态浓度。计算值准确预测了连续蓝光下的稳态气孔导度。
The stomatal response to blue light was analyzed with gas-exchange techniques in Commelina communis L. leaves by using high-fluence-rate short pulses. Pulses of blue light were given under a background of high-fluence-rate red light, which maintained photosynthesis at near saturation and stomatal conductance at a steady state. A single blue light pulse of 1-100 sec induced an increase in stomatal conductance, which peaked after 15 min and then returned to the initial steady-stte level within 50-60 min after the pulse. The response could be repeatedly induced in the same leaf. Red light pulses on a red background did not induce any comparable response. The stomatal response quantified by integrating the conductance increases after pulse application approached saturation with increasing pulse duration (t1/2 .apprxeq. 9 sec with 250 .mu.mol .cntdot. m-2 .cntdot. sec-1 of blue light). After a saturating pulse, sensitivity to a second pulse was restored slowly. This recovery response, quantified from the conductance increases caused by the two pulses, approached saturation with a t1/2 of .apprxeq. 9 min. These results were used to test a model in which a molecular component in the sensory transduction process is considered to exist in two interconvertible forms, A and B. If B is the physiologically active form inducing stomatal opening, then A is the inactive form. The A to B conversion is a light-induced reaction and the B to A conversion is a thermal reaction. Rate constants for these reactions were estimated from single- and double-pulse experiments (at a fluence rate of 250 .mu.mol .cntdot. m-2 .cntdot. sec-1, k1 = 0.075 sec-1; thermal rate constant kd = 0.0014 sec-1), allowing the calculation of steady-state concentration of B under continuous irradiation. The calculated values accurately predicted the steady-state stomatal conductances under continuous blue light.