Gut Microbiota-Derived Metabolite Signature in Suckling and Weaned Piglets
Gut Microbiota-Derived Metabolite Signature in Suckling and Weaned Piglets
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DOI：
10.1021/acs.jproteome.0c00745
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发表时间：
2021-01-01
影响因子：
4.4
通讯作者：
Combes, Sylvie
中科院分区：
文献类型：
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作者：
Beaumont, Martin;Cauquil, Laurent;Combes, Sylvie
肠道微生物区系在哺乳期到断奶期的肠道发育中起着关键作用。本研究的目的是分析哺乳和断奶仔猪肠道微生物区系代谢产物的产生。我们研究了在两个不同的哺乳农场饲养的仔猪，以及同一农场出生后第21天断奶的仔猪。对同一批仔猪在哺乳期(出生后13天)和断奶后2天(出生后23天)的粪便代谢组(H-1核磁共振)、微生物区系组成(16S rRNA基因扩增序列)及其预测功能(PICRUSt2)进行了分析。哺乳期间细菌代谢产物甲胺、二甲胺、身体碱、酪胺、腐胺、5-氨基戊酸酯、琥珀酸和3-(4-羟基苯丙酸)的相对浓度高于断奶后。相比之下，断奶后短链脂肪酸乙酸酯和丙酸的相对浓度高于哺乳期。母猪来源对某些细菌代谢物(丙酸和异丁酸)水平也有影响。在哺乳和断奶仔猪身上观察到的粪便代谢组特征与特定的微生物区系预测的功能、结构和多样性有关。肠道微生物区系衍生的代谢物在哺乳仔猪和断奶仔猪之间差异丰富(例如，短链脂肪酸和生物胺)，已知调节肠道健康。因此，识别哺乳和断奶仔猪的代谢组特征为开发促进健康的营养策略铺平了道路，目标是在生命早期产生细菌代谢物。
The gut microbiota plays a key role in intestinal development at the suckling-to-weaning transition. The objective of this study was to analyze the production of metabolites by the gut microbiota in suckling and weaned piglets. We studied piglets raised in two separate maternity farms and weaned at postnatal day 21 in the same farm. The fecal metabolome (H-1 nuclear magnetic resonance) and the microbiota composition (16S rRNA gene amplicon sequencing) and its predicted functions (PICRUSt2) were analyzed in the same piglets during the suckling period (postnatal day 13) and 2 days after weaning (postnatal day 23). The relative concentrations of the bacterial metabolites methylamine, dimethylamine, cadaverine, tyramine, putrescine, 5-aminovalerate, succinate, and 3-(4-hydroxyphenylpropionate) were higher during the suckling period than after weaning. In contrast, the relative concentrations of the short-chain fatty acids acetate and propionate were higher after weaning than during the suckling period. The maternity of origin of piglets also influenced the level of some bacterial metabolites (propionate and isobutyrate). The fecal metabolome signatures observed in suckling and weaned piglets were associated with specific microbiota-predicted functionalities, structure, and diversity. Gut microbiota-derived metabolites, which are differentially abundant between suckling and weaned piglets (e.g., short-chain fatty acids and biogenic amines), are known to regulate gut health. Thus, identification of metabolome signatures in suckling and weaned piglets paves the way for the development of health-promoting nutritional strategies, targeting the production of bacterial metabolites in early life.