Effects of hachimijiogan, tokishakuyakusan, keishibukuryogan, ninjinto and unkeito on estrogen and progesterone secretion in preovulatory follicles incubated in vitro.
Effects of hachimijiogan, tokishakuyakusan, keishibukuryogan, ninjinto and unkeito on estrogen and progesterone secretion in preovulatory follicles incubated in vitro.
复制标题
hachimijiogan、tokishakuyakusan、keishibukuryogan、ninjinto 和 unkeito 对体外培养的排卵前卵泡中雌激素和孕激素分泌的影响。
DOI：
10.1142/s0192415x91000107
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Usuki
中科院分区：
文献类型：
--
作者：
S. Usuki
将10周龄大鼠在发情间期16时30分-发情前期2时、发情前期11时0分和发情前期23时0分取出的卵巢卵泡与不同剂量的Hachimijiogan（HJ）、Tokishakuyakusan（TS）、Keishibukuryogan（KB）、Ninjinto（NT）和Unkeito（UT）孵育120分钟。测定培养液中雌二醇-17 β（E2）和孕酮的浓度。在LH峰之前，生长卵泡的TS和KB以及排卵前卵泡的HJ、TS和KB的E2浓度显著降低。相反，在LH峰之前，排卵前卵泡的孕酮水平随着HJ、TS、KB和UT而显著增加。这些结果表明，HJ，TS，KB或UT刺激排卵前卵泡LH峰分泌孕酮，但TS或KB抑制排卵前卵泡生长LH峰前E2分泌。
Ovarian follicles, removed from 10-week old rats at 1630 hours diestrus-2, 1100 and 2300 hours proestrus, were incubated for 120 minutes with various doses of Hachimijiogan (HJ), Tokishakuyakusan (TS), Keishibukuryogan (KB), Ninjinto (NT) and Unkeito (UT). The estradiol-17 beta (E2) and progesterone concentrations in the incubation medium were measured. The concentrations of E2 were significantly decreased with TS and KB by growing follicles and with HJ, TS and KB by preovulatory follicles before a LH surge. In contrast, the levels of progesterone were significantly increased with HJ, TS, KB and UT by preovulatory follicles before a LH surge. These results suggest that HJ, TS, KB or UT stimulates preovulatory follicles before a LH surge to secrete progesterone, but TS or KB suppresses E2 secretion by growing preovulatory follicles before a LH surge.