Molecular microscopy of labeled polynucleotides: stability of osmium atoms.
Molecular microscopy of labeled polynucleotides: stability of osmium atoms.
复制标题
标记多核苷酸的分子显微镜：锇原子的稳定性。
DOI：
10.1016/0022-2836(77)90134-6
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发表时间：
1977
影响因子：
5.6
通讯作者：
Michael Beer
中科院分区：
文献类型：
--
作者：
M. Cole;J. W. Wiggins;Michael Beer
在高分辨扫描电子显微镜下，观察了多核苷酸及其修饰的多核苷酸与四氧化三铯、联吡啶或二羧基联吡啶反应，每个核苷酸结合一个或两个Os原子的情况。显微照片显示，各个Os原子的构型由它们与核酸分子的特定化学键决定，其数量和平均间距与预期值一致。尽管在成像过程中，这些键被破坏，但在多核苷酸链的窄带内发现了许多Os原子，并且在同一区域的扫描之间移动不到5？这些结果表明，使用臭氧作为一种特殊的染料，可以获得分辨率为5到10？的大分子的结构信息。
Polynucleotides and modified polynucleotides reacted with osmium tetroxide and bipyridine or dicarboxybipyridine to bind one or two osmium atoms per nucleotide were viewed in a high-resolution scanning transmission electron microscope under conditions where the osmium atoms are visible. The micrographs show the individual osmium atoms in a configuration determined by their specific chemical bonding with the nucleic acid molecule and with a number and average spacing that is consistent with the expected value. Although the bonds are broken during the imaging, many osmium atoms are found to be contained within the narrow band of the polynucleotide strand and to move less than 5 Å between scans of the same area. These results suggest that it is possible to obtain structural information on macromolecules to a resolution of 5 to 10 Å using osmium as a specific stain.