Maturation-associated changes in internal distribution and intra-ovarian microdistribution of tetrodotoxin in the pufferfish Takifugu pardalis
Maturation-associated changes in internal distribution and intra-ovarian microdistribution of tetrodotoxin in the pufferfish Takifugu pardalis
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DOI：
10.1007/s12562-018-1209-2
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发表时间：
2018-07-01
影响因子：
1.9
通讯作者：
Arakawa, Osamu
中科院分区：
文献类型：
--
作者：
Gao, Wei;Kanahara, Yoko;Arakawa, Osamu
为了阐明河豚毒素（tetrodotoxin，TTX）在雌性东方鲀（Takifugu pardalis）体内的成熟累积特征，根据卵巢切片的组织学将2014年11月至12月采集自日本长崎县大村湾的东方鲀（Takifugu pardalis）分为4组：卵黄囊泡期（Yv）、卵黄球期（Yg）-1、Yg-2和Yg-3。通过液相色谱-质谱法对各组各组织中的TTX进行定量，结果显示卵巢中的TTX浓度[小鼠单位（MU）/g]从Yv增加到Yg-1，卵巢中的TTX含量（MU/个体）从Yg-1增加到Yg-3。在Yv中，皮肤中的TTX占体内总TTX的86%，而从Yg-1到Yg-3，卵巢TTX占总量的53-76%。在2015年2月至5月从同一海湾采集的雌性样本中，产卵后卵巢中的TTX含量显著下降，肝脏中的TTX含量增加。免疫组织化学观察表明，TTX主要定位于未成熟卵母细胞的核内，随着卵母细胞成熟，TTX分布于卵黄囊和卵黄球内，随着产卵的临近，TTX也有部分转移到卵膜上。
To clarify the maturation-associated accumulation profile of tetrodotoxin (TTX) in the female pufferfish Takifugu pardalis, specimens collected from Omura Bay, Nagasaki Prefecture, Japan, from November through December, 2014, were separated based on the histology of ovarian sections into four groups: yolk vesicle stage (Yv), yolk globule stage (Yg)-1, Yg-2, and Yg-3. TTX quantification in each tissue in each group by liquid chromatography-mass spectrometry revealed that the TTX concentration [mouse unit (MU)/g] in the ovary increased from Yv to Yg-1, and the TTX amount (MU/individual) in the ovary increased from Yg-1 through Yg-3. In Yv, TTX in the skin accounted for 86% of the total TTX in the body, whereas from Yg-1 through Yg-3, ovarian TTX accounted for 53-76% of the total. In female specimens collected from the same bay from February to May 2015, the TTX amount in the ovary markedly decreased after spawning, and the TTX amount in the liver increased. Immunohistochemical observation of the ovarian sections indicated that TTX localized mainly in the nucleus of immature oocytes, but then distributes to the yolk vesicles and yolk globules as maturation advances, and is partly transferred to the egg membrane as spawning approaches.