Differences in cytokine responses to Onchocerca volvulus extract and recombinant Ov33 and OvL3-1 proteins in exposed subjects with various parasitologic and clinical states
Differences in cytokine responses to Onchocerca volvulus extract and recombinant Ov33 and OvL3-1 proteins in exposed subjects with various parasitologic and clinical states
复制标题
DOI：
10.1086/517317
复制
发表时间：
1997-09-01
影响因子：
6.4
通讯作者：
Pogonka, T
中科院分区：
文献类型：
--
作者：
Brattig, N;Nietz, C;Pogonka, T
研究了全身性盘尾丝虫病（GEN）受试者、具有sowdah形式的受试者和具有暴露但没有盘尾丝虫病（地方性正常/脓毒症免疫; EN/PI）的受试者对盘尾丝虫提取物（OvAg）和重组Ov 33和OvL 3 -1蛋白的细胞因子应答。在sowdah和EN/PI中，响应OvAgs产生的细胞因子水平高于GEN受试者。外周血单个核细胞不产生干扰素-γ对抗原的反应。OvAg诱导白细胞介素（IL）-5、IL-2、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和可溶性IL-2受体。EN/PI和sowdah人产生的IL-5和IL-2显著高于GEN人，EN/PI人的GM-CSF水平显著高于GEN人。GEN受试者中的低IL-5和GM-CSF水平通过添加外源性IL-2而增加。Ov 33和OvL 3 -1刺激了IL-10的产生，而IL-5和IL-2的产生较少。研究组未显示严格的Th 2样细胞因子应答。
Subjects with generalized onchocerciasis (GEN), with the sowdah form, and with exposure but without onchocerciasis (endemic normal/putatively immune; EN/PI) were studied for cytokine responses to Onchocerca volvulus extract (OvAg) and recombinant Ov33 and OvL3-1 proteins. Higher levels of cytokines were produced in response to OvAgs in sowdah and EN/PI than in GEN subjects. Peripheral blood mononuclear cells did not produce interferon-gamma in response to antigens. OvAg induced interleukin (IL)-5, IL-2, granulocyte-macrophage colony-stimulating factor (GM-CSF), and soluble IL-2 receptor. EN/PI and sowdah persons produced significantly more IL-5 and IL-2 than GEN subjects, and EN/PI subjects had significantly higher GM-CSF levels than GEN persons. The low IL-5 and GM-CSF levels in GEN subjects were increased by addition of exogenous IL-2. Ov33 and OvL3-1 stimulated production of IL-10 and less IL-5 and IL-2. The study groups did not show a strict Th2-like cytokine response.