Catabolism of myo‐Inositol to Precursors Utilized for De Novo Glycerolipid Biosynthesis
Catabolism of myo‐Inositol to Precursors Utilized for De Novo Glycerolipid Biosynthesis
复制标题
肌醇分解代谢为用于从头甘油脂生物合成的前体
DOI：
--
复制
发表时间：
1985
影响因子：
4.7
通讯作者：
Robert E. Anderson
中科院分区：
文献类型：
--
作者：
S. Fliesler;P. Kelleher;Robert E. Anderson
在青蛙体内全身注射[2-~3H]肌醇后，超过一半的标记物被掺入视网膜视杆外节膜中的甘油脂类，而不是磷脂类。在甲醇分解和分离脂类的差示提取后，放射性主要在水相中恢复。磷脂酶C水解总膜脂后，97%的放射性可用有机溶剂提取，70%的标记在1，2-二甘油酯中。这些结果表明，该标记主要被结合到膜甘油脂的甘油基部分。青蛙眼内注射或青蛙视网膜与[2-~3H]肌醇体外孵育后，放射性几乎完全掺入视杆外节膜上的磷脂酰肌醇。视网膜与[U-14C]葡萄糖醛酸孵育不会导致标记的视网膜脂的形成。这些结果表明，肌醇可以系统地分解为用于视网膜甘油脂生物合成的前体。
Systemic injection of [2‐3H]myo‐inositol into frogs resulted in the incorporation of more than half of the label into glycerolipid classes other than phosphoinositides in retinal rod outer segment membranes. Following methanolysis and differential extraction of isolated lipid classes, radioactivity was recovered primarily in the aqueous phase. After phospholipase C hydrolysis of the total membrane lipids, 97% of the radioactivity was extractable with organic solvents, and 70% of the label in lipids was in 1,2‐diglycerides. These results indicate that the label was incorporated primarily into the glyceryl moiety of the membrane glycerolipids. Intraocular injection of frog eyes or in vitro incubation of frog retinas with [2‐3H]myo‐inositol resulted in the incorporation of radioactivity almost exclusively into phosphoinositides in rod outer segment membranes. Incubation of retinas with [U‐14C]glucuronic acid did not result in the formation of labeled retinal lipids. These results suggest that myo‐inositol can be catabolized systemically to precursors utilized for glycerolipid biosynthesis in the retina.
影响因子：
--
作者：
Wiegand,RD;Anderson,RE
通讯作者：
Anderson,RE