GATA4 is Involved in the Gonadal Development and Maturation of the Teleost Fish Tilapia, Oreochromis niloticus
GATA4 is Involved in the Gonadal Development and Maturation of the Teleost Fish Tilapia, Oreochromis niloticus
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GATA4 参与硬骨鱼罗非鱼 (Oreochromis niloticus) 的性腺发育和成熟
DOI：
10.1262/jrd.11-131s
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发表时间：
2012-04-01
影响因子：
1.8
通讯作者：
Nagahama, Yoshitaka
中科院分区：
文献类型：
--
作者：
Li, Jianzhong;Chen, Wenjuan;Nagahama, Yoshitaka
GATA4是GATA家族的一员，是一种众所周知的转录因子，涉及哺乳动物性别决定和性别分化的调节。然而，人们对GATA4在鱼类繁殖中的可能作用知之甚少。本研究从罗非鱼中克隆并鉴定了全长GATA4 cDNA。罗非鱼GATA4基因包含1179个核苷酸的开放阅读框（ORF），编码392个氨基酸的蛋白。测序结果表明，罗非鱼GATA4蛋白与其他脊椎动物具有较高的同源性（63.1% ~ 74.6%）。RT-PCR分析表明，GATA4基因在成年罗非鱼的卵巢、睾丸、肝脏、肠道和心脏均有表达。采用原位杂交技术研究了GATA4基因在罗非鱼性腺分化和发育过程中的时空表达规律。在未分化性腺中，GATA4在两性体细胞中均有表达。随后，GATA4在罗非鱼分化、幼鱼和成鱼的卵巢和睾丸中持续表达。我们的数据首次表明GATA4不仅对性腺分化的开始是必要的，而且对性腺的发育和成熟也很重要。
GATA4, a member of the GATA family, is a well-known transcription factor implicated in the regulation of sex determination and sexual differentiation in mammals. However, little is known about the possible role of GATA4 in fish reproduction. In the present study, a full-length GATA4 cDNA from the tilapia was cloned and characterized. The tilapia GATA4 gene contained an open reading frame (ORF) of 1179 nucleotides encoding a protein of 392 amino acids. Sequence alignment revealed that the tilapia GATA4 protein shared higher homology (ranging from 63.1 to 74.6%) with other vertebrates. RT-PCR analysis indicated that the GATA4 gene is expressed in the ovary, testis, liver, intestine and heart in adult tilapia. in situ hybridization was performed to examine the temporal and spatial expression patterns of GATA4 during tilapia gonadal differentiation and development. In the undifferentiated gonad, GATA4 was expressed in the somatic cells of both sexes. Subsequently, GATA4 expression persisted in the differentiated, juvenile and adult ovary and testis in tilapia. Our data indicate for the first time that GATA4 is not only necessary for the onset of gonadal differentiation, but also important for gonadal development and maturation.