Molecular weights of apoprotein B obtained from human low-density lipoprotein (apoprotein B-PI) and from rat very low density lipoprotein (apoprotein B-PIII).
Molecular weights of apoprotein B obtained from human low-density lipoprotein (apoprotein B-PI) and from rat very low density lipoprotein (apoprotein B-PIII).
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从人低密度脂蛋白（脱辅基蛋白 B-PI）和大鼠极低密度脂蛋白（脱辅基蛋白 B-PIII）获得的脱辅基蛋白 B 的分子量。
DOI：
10.1021/bi00327a042
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Puppione,DL
中科院分区：
文献类型：
--
作者：
Elovson,J;Jacobs,JC;Schumaker,VN;Puppione,DL
通过密度为 1.020 和 1.050 g/mL 之间的差速离心从单个供体中分离出人类低密度脂蛋白 (LDL)。 LDL 在 7 M 盐酸胍中被还原和烷基化，并通过用乙醚和乙醇的混合物在冷下多次提取来去除脂质。对所得人脱辅基蛋白 B（脱辅基蛋白 B-PI）在 6.00 M 盐酸胍中低浓度的沉降研究显示，25 C 时沉降系数为 2.15 ± 0.04 S 的单一尖锐边界，未校正粘度或密度。在分析超速离心机中以低速在相同溶剂中进行的扩散实验给出了 Z) 25= 0.694 ± 0.043 Fick。将这些值与 0.703 mL/g 的表观比容相结合，得到的分子量为 387000，与通过 7 M 盐酸胍沉降平衡分析获得的分子量没有区别。通过校准的沉降分析、盐酸胍中的琼脂糖2B色谱法以及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳也获得了类似的值。从 Triton WR1339 处理动物的血清中分离出的大鼠极低密度脂蛋白 (VLDL)，被用作大鼠脱辅基蛋白 B-PIII 的来源。将脱脂的VLDL溶解在十二烷基硫酸钠中，并通过Sepharose 4B层析分离脱辅基蛋白B-PIII。通过对密度和粘度进行适当校正，在分析超速离心后，大鼠脱辅基蛋白 B-PIII 在 6 和 7.7 M 盐酸胍中的行为是相同的，对应于 6 M 盐酸胍中的沉降系数和扩散系数分别为 1.47 S 和 0.92 Fick。这些数据可以组合起来得到210000的分子量。通过校准的沉降分析、通过在盐酸胍中的Sepharose 2B色谱法以及通过在十二烷基硫酸钠中的聚丙烯酰胺凝胶电泳获得类似的值。
Human low-density lipoproteins (LDL) were isolated from single donors by differential cen-trifugation between densities of 1.020 and 1.050 g/mL. The LDL were reduced and alkylated in 7 M guanidine hydrochloride, and the lipid was removed by multiple extractions in the cold with a mixture of diethyl ether and ethanol. Sedimentation studies on the resultant human apoprotein B (apoprotein B-PI) at low concentrations in 6.00 M guanidine hydrochloride showed a single sharp boundary with a sedimentation coefficient of 2.15 ą 0.04 S at 25 C, uncorrected for viscosity or density. Diffusion experiments performed in the same solvent at low speeds in the analytical ultracentrifuge gave a Z) 25= 0.694 ą 0.043 Fick. Combining these values with an apparent specific volume of 0.703 mL/g yielded a molecular weight of 387000, indistinguishable from that obtained by sedimentation equilibrium analysis in 7 M guanidine hydrochloride. Similar values were also obtained by calibrated sedimentation analysis, bySepharose 2B chromatography in guanidine hydrochloride, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Rat very low density lipoproteins (VLDL), isolated from sera of Triton WR1339 treated animals, were used as the source of rat apoprotein B-PIII. The delipidated VLDL were solubilized in sodium dodecyl sulfate, and apoprotein B-PIII was isolated by Sepharose 4B chromatography. With appropriate corrections for density and viscosity, the behavior of rat apoprotein B-PIII was identical, upon analytical ultracentrifugation, in 6 and 7.7 M guanidine hydrochloride, correspondingto sedimentation and diffusion coefficients of 1.47 S and 0.92 Fick, respectively, in 6 M guanidine hydrochloride. These data may be combined to yield a molecular weight of 210000. Similar values were obtained by calibrated sedimentation analysis, by Sepharose 2B chromatography in guanidine hydrochloride, and by polyacrylamide gel electrophoresis in sodium dodecyl sulfate.
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影响因子：
2.9
作者：
E. Reisler;H. Eisenberg
通讯作者：
H. Eisenberg
载脂蛋白 B 的溶解及其与低密度脂蛋白细胞受体的特异性结合。
DOI：
--
发表时间：
1977
影响因子：
11.1
作者：
R. Shireman;L. L. Kilgore;W. Fisher
通讯作者：
W. Fisher
浓缩氯化胍中蛋白质溶液的沉降平衡。
影响因子：
2.9
作者：
P. Munk;D. Cox
通讯作者：
D. Cox
载脂蛋白 B 的结构研究：与表面活性剂 Triton X-100 形成的复合物的可控异质性。
影响因子：
6.5
作者：
B. Patterson;L. L. Kilgore;P. Chun;W. Fisher
通讯作者：
W. Fisher
通过膜渗透压法检查盐酸胍中多链蛋白的解离。
影响因子：
2.9
作者：
Castellino Fj;R. Barker
通讯作者：
R. Barker