POSTTRANSCRIPTIONAL GENE-REGULATION AND SPECIFIC BINDING OF THE NONHISTONE PROTEIN HMG-I BY THE 3' UNTRANSLATED REGION OF BOVINE INTERLEUKIN-2 CDNA
POSTTRANSCRIPTIONAL GENE-REGULATION AND SPECIFIC BINDING OF THE NONHISTONE PROTEIN HMG-I BY THE 3' UNTRANSLATED REGION OF BOVINE INTERLEUKIN-2 CDNA
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DOI：
10.1073/pnas.84.18.6531
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发表时间：
1987-09-01
影响因子：
11.1
通讯作者：
JOHNSON, KR
中科院分区：
文献类型：
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作者：
REEVES, R;ELTON, TS;JOHNSON, KR
牛白细胞介素2 (il -2) cDNA的3“未翻译尾区（3”-UTR）连接到细菌编码氯霉素乙酰转移酶（CAT）的“标记”基因的3“末端，在体内作为淋巴细胞特异性基因调控元件，通过转染将杂交融合基因引入牛淋巴细胞。也有证据表明，3''-UTR及其保守（TATT）n基序可能在淋巴细胞中具有多种功能，在染色体水平上，该序列可能参与非组蛋白染色质高迁移率组蛋白hmm - i的特异性结合，在RNA水平上，保守序列参与淋巴细胞特异性核酸酶选择性转录后mRNA降解。这些结果表明，bIL-2 cDNA的3''-UTR及其保守（TATT）n序列在体内具有复杂的作用。它们也为哺乳动物的许多其他免疫反应和生长调节基因的3”- utr中相似的富含a + t序列的高度保守性提供了一个合理的解释。
The 3'' untranslated tail region (3''-UTR) of the cDNA of bovine interleukin 2 (bIL-2) acts as a lymphoid cell-specific gene regulatory element in vivo when ligated to the 3'' end of the "marker" bacterial gene coding for chloramphenicol acetyltransferase (CAT) and the hybrid fusion gene is introduced into bovine lymphoid cells by transfection. Evidence is also presented that the 3''-UTR with its conserved (TATT)n motif probably has multiple functions in lymphoid cells operating both at the chromosomal level, where the sequence may be involved in the specific binding of the nonhistone chromatin high mobility group protein HMG-I, and at the RNA level, where the conserved sequence is involved in selective posttranscriptional mRNA degradation by a lymphocyte-specific nuclease(s). These results suggest a complex in vivo role for the 3''-UTR of bIL-2 cDNA and the conserved (TATT)n sequences found within it. They also offer a plausible explanation for the high degree of conservation of similar A+T-rich sequences in the 3''-UTRs of many of the other immune-response and growth-regulatory genes of mammals.