Multicentre evaluations of two new rapid IgG4 tests (WB rapid and panLF rapid) for detection of lymphatic filariasis.
Multicentre evaluations of two new rapid IgG4 tests (WB rapid and panLF rapid) for detection of lymphatic filariasis.
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DOI：
10.1186/1475-2883-6-9
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发表时间：
2007-10-26
期刊：
影响因子：
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通讯作者：
Weerasooriya, Mirani
中科院分区：
文献类型：
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作者：
Noordin, Rahmah;Itoh, Makoto;Weerasooriya, Mirani
在消除淋巴丝虫病的全球努力中，快速现场适用检测是有用的工具，可在偏远地区进行现场检测，并迅速获得结果。完全依赖现有的几种测试可能会危及LF消除计划的进展，因此，引入其他快速测试将有助于解决这一问题。两种新的快速免疫层析IgG 4检测试剂盒，即WB rapid和panLF rapid，分别用于检测班氏丝虫病和所有三种淋巴丝虫。使用BmSXP重组抗原开发WB rapid，而使用BmR 1和BmSXP重组抗原开发PanLF rapid。USM分别对165份WB快速检测和276份panLF快速检测进行了评估，其余的被送往马来西亚的另一所大学（98 WB快速，129 panLF快速）和印度尼西亚的大学（56例WB快速，62例panLF快速），日本（每项试验152例）和印度（每项试验18例），其中每项试验均以设盲方式进行独立评价。WB rapid和panLF rapid的平均敏感性分别为97.6%（94%~ 100%）和96.5%（94%~ 100%），平均特异性均为99.6%（99%~ 100%）。因此，这项研究表明，这两种IgG 4快速检测是高度敏感和特异性的，将是有用的额外的测试，以促进全球消除这种疾病的努力。
In the global effort to eliminate lymphatic filariasis (LF), rapid field-applicable tests are useful tools that will allow on-site testing to be performed in remote places and the results to be obtained rapidly. Exclusive reliance on the few existing tests may jeopardize the progress of the LF elimination program, thus the introduction of other rapid tests would be useful to address this issue. Two new rapid immunochromatographic IgG4 cassette tests have been produced, namely WB rapid and panLF rapid, for detection of bancroftian filariasis and all three species of lymphatic filaria respectively. WB rapid was developed using BmSXP recombinant antigen, while PanLF rapid was developed using BmR1 and BmSXP recombinant antigens. A total of 165 WB rapid and 276 panLF rapid tests respectively were evaluated at USM and the rest were couriered to another university in Malaysia (98 WB rapid, 129 panLF rapid) and to universities in Indonesia (56 WB rapid, 62 panLF rapid), Japan (152 of each test) and India (18 of each test) where each of the tests underwent independent evaluations in a blinded manner. The average sensitivities of WB rapid and panLF rapid were found to be 97.6% (94%-100%) and 96.5% (94%-100%) respectively; while their average specificities were both 99.6% (99%-100%). Thus this study demonstrated that both the IgG4 rapid tests were highly sensitive and specific, and would be useful additional tests to facilitate the global drive to eliminate this disease.