Antimicrobial drug susceptibility of Staphylococcus aureus strains isolated from bovine and ovine mammary glands.
Antimicrobial drug susceptibility of Staphylococcus aureus strains isolated from bovine and ovine mammary glands.
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从牛和羊乳腺分离的金黄色葡萄球菌菌株的抗菌药物敏感性。
DOI：
10.3168/jds.s0022-0302(03)73917-4
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发表时间：
2003
影响因子：
3.5
通讯作者：
S. Cerú
中科院分区：
文献类型：
--
作者：
A. Pengov;S. Cerú
所选抗菌剂对金黄色葡萄球菌的活性通过琼脂纸片扩散试验确定抑制区域的直径，并通过 E 试验确定最小抑菌浓度 (MIC)。本研究中使用的 92 株金黄色葡萄球菌菌株是从牛 (n = 76) 和绵羊 (n = 16) 的乳房内感染中分离出来的。选择了四种常用于乳腺炎治疗的抗生素：青霉素-G、氨苄青霉素、卡那霉素和头孢氨苄。第五种化合物（苯唑西林）用于检测耐甲氧西林金黄色葡萄球菌，但没有发现此类菌株。根据评价标准，65.2%（青霉素）至93.5%（卡那霉素、头孢氨苄）金黄色葡萄球菌菌株对受试抗生素敏感。绵羊金黄色葡萄球菌菌株的耐药率低于牛菌株。两种药敏试验结果的比较表明，除青霉素和氨苄青霉素外，结果令人满意。分析 MIC 终点和区域直径值的结果，根据 Metzler 和 DeHaan 的错误率有界方法，青霉素的错误率为 3.3%，氨苄青霉素的错误率为 3.8%，发生了非常大的错误。
The activity of selected antimicrobial agents against Staphylococcus aureus was determined with the agar disk diffusion test to determine the diameter of the zone of inhibition and the E-test for determination of the minimal inhibitory concentration (MIC). The 92 S. aureus strains used in this study were isolated from bovine (n = 76) and ovine (n = 16) intramammary infections. Four antibiotics, which are frequently used in mastitis therapy were chosen: penicillin-G, ampicillin, kanamycin, and cephalexine. The fifth compound (oxacillin) was used to detect methicillin-resistant S. aureus, but no such strains could be found. According to the evaluation criteria, 65.2 (penicillin) to 93.5% (kanamycin, cephalexine) S. aureus strains were susceptible to the antibiotics tested. Ovine S. aureus strains reveal a lower resistance rate than bovine isolates. Comparison of the results of the two methods of susceptibility testing shows, with exception of penicillin and ampicillin, satisfactory agreement. Analyzing the results of the MIC endpoints and the zone diameter values, very major errors, according to the error rate bounded method of Metzler and DeHaan, occurred at an error rate of 3.3% for penicillin and 3.8% for ampicillin.