A capillary dielectrophoretic chip for real-time blood cell separation from a drop of whole blood
A capillary dielectrophoretic chip for real-time blood cell separation from a drop of whole blood
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DOI：
10.1063/1.4802269
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发表时间：
2013-03-01
期刊：
影响因子：
3.2
通讯作者：
Chang, Hsien-Chang
中科院分区：
文献类型：
--
作者：
Liao, Shu-Hsien;Chang, Ching-Yu;Chang, Hsien-Chang
本研究提出了一种毛细管电泳片，利用负电泳力从一滴全血(约1亩L)中分离血细胞。通过分析介电泳力处理前后的图像来评价分离效率。不同血球压积(10%-60%)的血液样本用不同的分离电压和芯片设计进行测试。在本研究中，一个50微米间隙设计的芯片在30 S内，当红细胞压积在10%-50%范围内时，分离效率约为90%。此外，在操作后，通过分离电极来电化学测量葡萄糖浓度。分离血细胞后，电流响应显著增加(8.8倍)，这不仅归因于血细胞分离，也归因于介电泳力对样品的干扰。(C)2013年作者(S)。除另有说明外，所有文章内容均按照知识共享署名3.0未移植许可进行许可。[http://dx.doi.org/10.1063/1.4802269]
This study proposes a capillary dielectrophoretic chip to separate blood cells from a drop of whole blood (approximately 1 mu l) sample using negative dielectrophoretic force. The separating efficiency was evaluated by analyzing the image before and after dielectrophoretic force manipulation. Blood samples with various hematocrits (10%-60%) were tested with varied separating voltages and chip designs. In this study, a chip with 50 mu m gap design achieved a separation efficiency of approximately 90% within 30 s when the hematocrit was in the range of 10%-50%. Furthermore, glucose concentration was electrochemically measured by separating electrodes following manipulation. The current response increased significantly (8.8-fold) after blood cell separation, which was attributed not only to the blood cell separation but also to sample disturbance by the dielectrophoretic force. (C) 2013 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License. [http://dx.doi.org/10.1063/1.4802269]