One-step synthesis of N-doped carbon dots, and their applications in curcumin sensing, fluorescent inks, and super-resolution nanoscopy
One-step synthesis of N-doped carbon dots, and their applications in curcumin sensing, fluorescent inks, and super-resolution nanoscopy
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N掺杂碳点的一步合成及其在姜黄素传感、荧光墨水和超分辨率纳米显微镜中的应用
DOI：
10.1007/s00604-019-3762-5
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发表时间：
2019-10-01
影响因子：
5.7
通讯作者：
Huang, Jing
中科院分区：
文献类型：
--
作者：
Bu, Lingli;Luo, Tao;Huang, Jing
采用一步水热法制备了荧光激发/发射峰位于365/450 nm的氮掺杂碳点。这些点具有显著的光稳定性、荧光闪烁和良好的生物相容性，这有利于在随机光学重建显微镜(STORM)中的应用。用647 nm激光激发对HeLa细胞成像时，空间分辨率可达~60 nm。这为基于随机光开关的各种超分辨率技术开辟了新的可能性。N-CD的显著光学性质也使它们能够作为隐形安全油墨应用于图案化、信息存储和防伪。进一步研究发现，由于内滤波效应和静态猝灭的共同作用，姜黄素可以高效地猝灭N-CdS的荧光。在此基础上，建立了测定姜黄素的荧光分析方法，检出限为21 ng/m L−1。图形摘要N-掺杂碳点(N-Cd)的应用示意图。姜黄素高效猝灭N-硫化镉的荧光。N-Cd的显著光学性质使其在荧光油墨、细胞成像和随机光学重建显微镜(STORM)中得到应用。
Nitrogen-doped carbon dots (N-CDs) with fluorescence excitation/emission maxima at 365/450 nm were prepared by a one-step hydrothermal approach. The dots possess remarkable photostability, fluorescence blinking and good biocompatibility, and this favors utilization in stochastic optical reconstruction microscopy (STORM). A spatial resolution down to ~60 nm was achieved when imaging HeLa cells using 647-nm laser excitation. This opens new possibilities for various super-resolution techniques based on stochastic optical switching. The remarkable optical properties of the N-CDs also enable them to be applied as invisible security ink for use in patterning, information storage and anti-counterfeiting. Further, it is found that the fluorescence of the N-CDs can be quenched by curcumin with high efficiency due to a combination of inner filter effect and static quenching. Based on this, a fluorometric assay with a detection limit of 21 ng mL−1was developed for the determination of curcumin.Graphical abstractSchematic representation of the applications of N-doped carbon dots (N-CDs). Curcumin quenches the fluorescence of N-CDs with high efficiency. The remarkable optical properties of the N-CDs enable them to be applied in fluorescent ink, cell imaging and stochastic optical reconstruction microscopy (STORM).