Purification of kidney epithelial cell growth inhibitors.
Purification of kidney epithelial cell growth inhibitors.
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肾上皮细胞生长抑制剂的纯化。
DOI：
10.1073/pnas.77.10.5989
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发表时间：
1980
影响因子：
11.1
通讯作者：
Armour,R
中科院分区：
文献类型：
--
作者：
Holley,RW;Böhlen,P;Fava,R;Baldwin,JH;Kleeman,G;Armour,R
从非洲绿色猴肾上皮细胞BSC-1细胞的培养液中分离到两种高分子量的生长抑制剂。在ng/ml浓度下，纯化的肾上皮细胞生长抑制剂可逆地阻止BSC-1细胞在细胞周期的G1期的生长。他们的行动是有选择性的;它们对BSC-1细胞最具活性，作为大鼠肺和人乳腺上皮细胞生长的抑制剂活性较低，并且不抑制培养物中3 T3小鼠胚胎成纤维细胞和人皮肤成纤维细胞的生长。它们对BSC-1细胞培养物的生长抑制作用被表皮生长因子或小牛血清抵消。
Two high molecular weight growth inhibitors have been isolated from the culture medium of BSC-1 cells, epithelial cells of African green monkey kidney. The purified kidney epithelial cell growth inhibitors, at ng/ml concentrations, reversibly arrest the growth of BSC-1 cells in the G1 phase of the cell cycle. Their action is selective; they are most active on BSC-1 cells, are less active as inhibitors of the growth of rat lung and human breast epithelial cells, and do not inhibit the growth of 3T3 mouse embryo fibroblasts ad human skin fibroblasts in culture. Their growth inhibitory action on BSC-1 cell cultures is counteracted by epidermal growth factor or calf serum.