IN-VIVO PROLIFERATIVE PATTERN OF TREMBLER HYPOMYELINATING SCHWANN-CELLS IS MODIFIED IN CULTURE - AN EXPERIMENTAL-ANALYSIS
IN-VIVO PROLIFERATIVE PATTERN OF TREMBLER HYPOMYELINATING SCHWANN-CELLS IS MODIFIED IN CULTURE - AN EXPERIMENTAL-ANALYSIS
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DOI：
10.1159/000111311
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发表时间：
1993-01-01
影响因子：
2.9
通讯作者：
RESSOUCHES, A
中科院分区：
文献类型：
--
作者：
DOTHI, NA;KOENIG, HL;RESSOUCHES, A
Trembler小鼠是一种雪旺细胞突变小鼠，其特征是周围神经的严重髓鞘形成不足、雪旺细胞高度增殖和周围存在多层基底膜。与其连续的体内分裂相反，从15天坐骨神经制备的突变体雪旺细胞在细胞培养中显示出比正常雪旺细胞更低的增殖率。然而，静止的Trembler Schwann细胞仍然能够像正常的Schwann细胞一样对外源性有丝分裂原如神经提取物和富含髓鞘的组分作出反应。此外，正常和TremblerSchwann细胞都响应于Trembler血清而增殖。成纤维细胞生长因子不是刺激体内突变体雪旺细胞增殖的促有丝分裂因子，因为它在Trembler血清中不存在，并且在Trembler成体坐骨神经中浓度很低。我们的研究结果表明，在体内，Trembler小鼠的血清中可能含有促有丝分裂因子，尚未表征，这可能会引发永久分裂的突变雪旺细胞，在正常小鼠的神经中的这些细胞的静止状态。
Trembler mouse, a Schwann cell mutation, is characterized by severe hypomyelination of peripheral nerves, high Schwann cell proliferation and the presence of a multilayered basal lamina which surrounds them. In contrast with their continuous in vivo division, mutant Schwann cells prepared from 15-day sciatic nerves display a lower proliferation rate in cell culture than normal Schwann cells. However, quiescent Trembler Schwann cells are still able to respond, as normal Schwann cells, to exogenous mitogens, such as nerve extracts and myelin-enriched fractions. In addition, both normal and Trembler Schwann cells proliferate in response to Trembler serum. Fibroblast growth factor is not the mitogenic factor which stimulates mutant Schwann cell proliferation in vivo, since it is absent in Trembler serum and poorly concentrated in Trembler adult sciatic nerves. Our results suggest that, in vivo, the serum of Trembler mouse probably contains mitogenic factors, not yet characterized, which may trigger the permanent division of mutant Schwann cells, in contrast to the quiescent state of these cells in the nerves of normal mice.