Chromosomal effects on peptidase activities in Drosophila melanogaster.
Chromosomal effects on peptidase activities in Drosophila melanogaster.
复制标题
染色体对果蝇肽酶活性的影响。
DOI：
10.1007/bf02399818
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发表时间：
1993
影响因子：
2.4
通讯作者：
Shalish,CI
中科院分区：
文献类型：
--
作者：
Hiraizumi,K;Mathes,KD;Shalish,CI
以果蝇（Drosophilamelanogaster）的肽酶系统（二肽酶-A、-B、-C和亮氨酸氨肽酶G、P）为模型，研究修饰基因对功能相似酶活性的影响。X，第二，第三染色体置换等基因系的屏幕显示存在的活性修饰剂的所有三条染色体上的肽酶。相关性分析表明，一些肽酶活性之间的协变是独立的遗传背景，而其他人与可变的第二染色体。还检测了部分纯化的肽酶的Km、Vmax和比活性的染色体特异性效应。此外，一个可重复的技术，使用阴离子交换柱色谱允许表征可能的两个假定的肽酶，甘氨酰-L-异亮氨酸-酶和L-亮氨酰-L-脯氨酸-酶，其动力学性质不同的二肽酶和亮氨酸氨基肽酶。这些发现证实了肽酶的活性调节剂的存在，就像果蝇中的其他酶一样。
The peptidase system inDrosophila melanogaster(dipeptidase-A, -B, and -C and leucine aminopeptidases G and P) was used as a model to study the effects of modifier genes on activity of enzymes with similar functions. A screen of X, second, and third chromosome substitution isogenic lines revealed the presence of activity modifiers for peptidases on all three chromosomes. Correlation analyses indicated that covariation between some of the peptidase activities is independent of genetic background, while others are associated with variable second chromosomes. Chromosome-specific effects onKm,Vmax, and specific activity of partially purified peptidases were also detected. Moreover, a repeatable technique using anion-exchange column chromatography allowed the characterization of possibly two putative peptidic enzymes, glycyl-l-isoleucine-ase andl-leucyl-l-proline-ase, whose kinetic properties differ from the dipeptidases and the leucine aminopeptidases. These findings confirm the existence of activity modifiers for peptidases, much like other enzymes inDrosophila melanogaster.