Thermogymnomonas acidicola gen. nov., sp nov., a novel thermoacidophilic, cell wall-less archaeon in the order Thermoplasmatales, isolated from a solfataric soil in Hakone, Japan
Thermogymnomonas acidicola gen. nov., sp nov., a novel thermoacidophilic, cell wall-less archaeon in the order Thermoplasmatales, isolated from a solfataric soil in Hakone, Japan
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DOI：
10.1099/ijs.0.65203-0
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发表时间：
2007-11-01
影响因子：
2.8
通讯作者：
Takashina, Tomonori
中科院分区：
文献类型：
--
作者：
Itoh, Takashi;Yoshikawa, Naoto;Takashina, Tomonori
从日本箱根 Ohwaku-dani 的硫磺田中分离出一种新型嗜热嗜酸、无细胞壁古细菌菌株 IC-189(T)。这些细胞是不规则的球菌，有时呈叶状、棒状或链状，大小变化很大，直径范围为0.8至8.0μm。该菌株在 38-68 摄氏度（最佳 60 摄氏度）和 pH 1.8-4.0（最佳 pH 3.0 左右）的温度范围内生长。菌株IC_189(T)是专性需氧和异养的，需要酵母提取物生长。酵母提取物、葡萄糖和甘露糖充当碳和能源。极性脂质主要由环状或无环甘油-双二植烷基-甘油四醚组成，主要的醌是具有七个类异戊二烯单元的甲基萘醌(MK-7)。总DNA的G+C含量为56.1mol%。 16S rRNA基因序列分析显示，菌株IC-189（T）属于热原体目（Thermoplasmatales），但与迄今为止已知的热原体属（Thermoplasma）、嗜苦菌属（Picrophilus）和铁原体属（Ferroplasma）的物种不同（序列相似性为86.2-91.0%）。这些表型和系统发育特性清楚地支持该菌株的单独分类地位。因此，菌株IC-189(T)代表了一个新的属（热原体目）和种，其名称为Thermogymnomonas Acidola gen。十一月，SP。十一月建议使用菌株 IC-189(T) (=JCM 13583(T) =DSM 18835(T))。
A novel thermoacidophilic, cell wall-less archaeon, strain IC-189(T), was isolated from a solfataric field in Ohwaku-dani, Hakone, Japan. The cells were irregular cocci, sometimes lobed, club-shaped or catenated, and were highly variable in size, ranging from 0.8 to 8.0 mu m in diameter. The strain grew at temperatures in the range 38-68 degrees C (optimally at 60 degrees C) and at pH 1.8-4.0 (optimally at around pH 3.0). Strain IC_189(T) was obligately aerobic and heterotrophic, requiring yeast extract for growth. Yeast extract, glucose and mannose served as carbon and energy sources. The polar lipids consisted mainly of cyclic or acyclic glycerol-bisdiphytanyl-glycerol tetraethers, and the predominant quinone was a menaquinone with seven isoprenoid units (MK-7). The G +C content of total DNA was 56.1 mol%. 16S rRNA gene sequence analysis revealed that strain IC-189(T) was a member of the order Thermoplasmatales, but diverged from the hitherto known species of the genera Thermoplasma, Picrophilus and Ferroplasma (86.2-91.0% sequence similarity). These phenotypic and phylogenetic properties clearly support a separate taxonomic status for this strain. Therefore, strain IC-189(T) represents a novel genus (order Thermoplasmatales) and species, for which the name Thermogymnomonas acidicola gen. nov., sp. nov. is proposed, with type strain IC-189(T) (=JCM 13583(T) =DSM 18835(T)).