COMPARISON OF AN AVIDIN-BIOTIN IMMUNOASSAY WITH 3 COMMERCIALLY AVAILABLE IMMUNOFLUORESCENCE KITS FOR TYPING OF HERPES-SIMPLEX VIRUS
COMPARISON OF AN AVIDIN-BIOTIN IMMUNOASSAY WITH 3 COMMERCIALLY AVAILABLE IMMUNOFLUORESCENCE KITS FOR TYPING OF HERPES-SIMPLEX VIRUS
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DOI：
10.1136/jcp.38.10.1158
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发表时间：
1985-01-01
影响因子：
3.4
通讯作者：
RICHARDS, DF
中科院分区：
文献类型：
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作者：
BARNARD, DL;JOHNSON, FB;RICHARDS, DF
比较了亲和素-生物素复合物系统与三种市售免疫荧光试剂盒对临床标本中单纯疱疹病毒分离株的血清分型。灵敏度值显示Electro-Nucleonics和Immulok试剂可用于检测病毒的存在，而预测值显示Syva和Immulok试剂在单纯疱疹病毒血清型之间具有足够的区分力。抗生物素蛋白-生物素复合物系统在检测细胞培养物中的单纯疱疹病毒抗原和单纯疱疹病毒分离株的血清分型方面均等同于或上级于所测试的免疫荧光试剂。
An avidin-biotin complex system was compared with three commercially available immunofluorescence kits for serotyping herpes simplex virus isolates from clinical specimens. Sensitivity values showed that the Electro-Nucleonics and Immulok reagents were useful in detecting the presence of virus, whereas the predictive values showed that the Syva and Immulok reagents possessed adequate discrimination between the herpes simplex virus serotypes. The avidin-biotin complex system was equal or superior to the immunofluorescence reagents tested both in detecting herpes simplex virus antigens in cell culture and in serotyping herpes simplex virus isolates.