Transgenic Cry1Ab rice does not impact ecological fitness and predation of a generalist spider.
Transgenic Cry1Ab rice does not impact ecological fitness and predation of a generalist spider.
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DOI：
10.1371/journal.pone.0035164
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ye GY
中科院分区：
文献类型：
--
作者：
Tian JC;Chen Y;Li ZL;Li K;Chen M;Peng YF;Hu C;Shelton AM;Ye GY
在中国，表达苏云金芽孢杆菌（Bt）Cry1Ab蛋白的转基因水稻的商业化释放是一个有争议的话题。争论的一个主要焦点是Bt水稻对非靶标生物的生态安全性，特别是帮助控制害虫种群的捕食者和寄生虫。采用三重营养生物测定法研究了转Cry1Ab基因水稻对捕食性地蜘蛛（Pardosa pseudoannulata（Bösenberg et Strand））适合度参数的影响。取食Bt稻和非Bt稻的蜘蛛的存活率、发育时间和繁殖力没有显著差异。此外，ELISA和PCR肠道测定，以及功能反应试验表明，捕食P.pseudoannulata在Bt水稻和非Bt水稻田没有显着差异。转Cry1Ab基因水稻品系在实验室条件下对拟环纹瓢虫的存活、发育时间和繁殖力没有影响，在田间条件下对拟环纹瓢虫的捕食作用也没有影响。这表明，这种重要的捕食者不会受到伤害，如果转基因水稻的商业化。
The commercial release of rice genetically engineered to express a Cry1Ab protein from Bacillus thuringiensis (Bt) for control of Lepidoptera in China is a subject of debate. One major point of the debate has focused on the ecological safety of Bt rice on nontarget organisms, especially predators and parasitoids that help control populations of insect pests. A tritrophic bioassay was conducted to evaluate the potential impact of Cry1Ab-expressing rice on fitness parameters of a predaceous ground spider (Pardosa pseudoannulata (Bösenberg et Strand)) that had fed on Bt rice-fed brown planthopper (Nilaparvata lugens (Stål)) nymphs. Survival, development time and fecundity of this spider were not different when they were fed with Bt rice-fed or non-Bt rice-fed prey. Furthermore, ELISA and PCR gut assays, as well as a functional response trial, indicated that predation by P. pseudoannulata was not significantly different in Bt rice or non-Bt rice fields. The transgenic Cry1Ab rice lines tested in this study had no adverse effects on the survival, developmental time and fecundity of P. pseudoannulata in the laboratory or on predation under field conditions. This suggests that this important predator would not be harmed if transgenic Cry1Ab rice were commercialized.