Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model.
Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model.
复制标题
载脂蛋白E小鼠模型缺血后肢移植同种异体脂肪源性再生细胞的动态成像
DOI：
10.2147/ijn.s118328
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发表时间：
2017
影响因子：
8
通讯作者：
Lu X
中科院分区：
文献类型：
--
作者：
Zheng Y;Qin J;Wang X;Peng Z;Hou P;Lu X
同种异体脂肪源性再生细胞（ADRCs）移植是治疗严重缺血性疾病的一种很有前途的方法。然而，关于ADRC的体内作用、命运和迁移以及其治疗性血管生成机制的信息很少。在这项研究中，获得了表达绿色荧光蛋白的ADRCs（GFP-ADRCs），用乙酰化3-氨丙基三甲氧基硅烷（APTS）包裹的氧化铁纳米颗粒（APTS NPs）标记，并注射到老年载脂蛋白E敲除（ApoE-KO）小鼠模型与后肢缺血。然后，进行3.0T磁共振成像（MRI）以动态追踪靶向后肢缺血的ADRCs在ApoE-KO小鼠模型中的作用。在4周的随访期间，通过连续3.0 T MRI扫描，标记的细胞在缺血肌肉中被可视化为大的低信号点。通过普鲁士蓝染色和荧光显微镜检查证实死后标本中存在标记的GFP-ADRCs。这项研究表明，基于ADRC移植的有效性和可行性以及可用的组织量，同种异体ADRC为严重缺血性疾病的治疗性血管生成提供了巨大的潜在应用。
Transplantation of allogeneic adipose-derived regenerative cells (ADRCs) is a promising treatment modality for severe ischemic diseases. However, minimal information is available on the in vivo effects, fate, and migration of ADRCs, as well as the mechanisms of their therapeutic angiogenesis. In this study, green fluorescent protein-expressing ADRCs (GFP-ADRCs) were obtained, labeled with acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (APTS NPs), and injected into an old apolipoprotein E knockout (ApoE-KO) mouse model with hind limb ischemia. Then, 3.0 T magnetic resonance imaging (MRI) was performed to dynamically trace the role of ADRCs targeting hind limb ischemia in the ApoE-KO mice model. Labeled cells were visualized as large hypointense spots in ischemic muscles by serial 3.0 T MRI scans during a 4-week follow-up. The presence of labeled GFP-ADRCs was confirmed by Prussian blue staining and fluorescence microscopy on postmortem specimens. This study showed that allogeneic ADRCs offer great potential application for therapeutic angiogenesis in severe ischemic disease based on the efficacy and feasibility of ADRC transplantation and on the available amounts of tissue.