Aqueous Angiography: Aqueous Humor Outflow Imaging in Live Human Subjects
Aqueous Angiography: Aqueous Humor Outflow Imaging in Live Human Subjects
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DOI：
10.1016/j.ophtha.2017.03.058
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发表时间：
2017-08-01
期刊：
影响因子：
13.7
通讯作者：
Weinreb, Robert N.
中科院分区：
文献类型：
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作者：
Huang, Alex S.;Camp, Andrew;Weinreb, Robert N.
微创小梁旁路/消融青光眼手术已显示眼内压（IOP）降低，但幅度可变且不可预测。通过了解个体患者的房水流出（AHO）途径的生理学，可以提高IOP降低的幅度及其可预测性。房水血管造影是一种前段血管造影方法，其成像AHO 1 -4类似于后段静脉血管造影如何可视化视网膜血流。在多个物种的摘除眼1 -3和活体非人灵长类动物（NHP）的完整眼4中，眼动脉血管造影显示节段性（非360度均匀）AHO。在NHP中观察到脉动和动态AHO，其中具有/不具有血管造影AHO的区域可以分别以实时方式丢失/获得血管造影信号4。在这里，我们展示了第一次水血管造影图像从生活人类受试者在超声乳化白内障吸除术。将吲哚菁绿色（ICG）作为囊膜染色剂5引入前房，同时作为房水血管造影成像的示踪剂。
Minimally invasive trabecular bypass/ablation glaucoma surgeries have shown intraocular pressure (IOP) reduction, but the magnitude is variable and unpredictable. By understanding the physiology of aqueous humor outflow (AHO) pathways of individual patients, it may be possible to enhance the magnitude of IOP lowering and its predictability.Aqueous angiography is an anterior-segment angiographic method that images AHO1–4 similar to how intravenous angiography for the posterior segment visualizes retinal blood flow. Aqueous angiography has shown segmental (non-360-uniform) AHO in enucleated eyes from multiple species1–3 and in intact eyes from living non-human primates (NHP) 4. Pulsatile and dynamic AHO were seen in NHPs where regions with/without angiographic AHO could lose/gain angiographic signal respectively in a real-time fashion4. Here, we demonstrate the first aqueous angiographic images from living human subjects obtained during phacoemulsification. Indocyanine green (ICG) was introduced into the anterior chamber as a capsular stain5 while simultaneously serving as a tracer for aqueous angiographic imaging.