Stimulatory effect of hydrothermally synthesized biodegradable hydroxyapatite granules on osteogenesis and direct association with osteoclasts
Stimulatory effect of hydrothermally synthesized biodegradable hydroxyapatite granules on osteogenesis and direct association with osteoclasts
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DOI：
10.1016/j.biomaterials.2009.05.002
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发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Ikeda, Tohru
中科院分区：
文献类型：
--
作者：
Gonda, Yoshinori;Ioku, Koji;Ikeda, Tohru
采用水热法制备了具有独特球形的缺钙羟基磷灰石（HA）颗粒。还通过正常烧结制备了球形化学计量的HA颗粒，并将两种颗粒植入大鼠胫骨中以比较对每种植入物的生物学反应。植入后12周和24周，缺钙HA颗粒的体积明显小于化学计量的HA颗粒，并且缺钙HA颗粒的生物降解性得到证实。与化学计量HA相比，缺钙HA植入区域的破骨细胞数量更多，成骨细胞表面更大，骨体积更大，表明缺钙HA的骨吸收影响该区域的成骨。为了分析破骨细胞对骨生成的直接贡献，将C2 C12多能成肌细胞（其具有在骨形态发生蛋白2存在下分化成成骨细胞的潜力）与在钙缺乏的HA、化学计量的HA、P-磷酸三钙盘或塑料皿上培养的破骨细胞的上清液或在塑料皿上培养的骨髓巨噬细胞一起培养。破骨细胞上清液刺激C2 C12细胞中Runx 2和骨钙素的表达，但骨髓巨噬细胞不刺激Runx 2和骨形成蛋白2的表达。破骨细胞培养上清刺激碱性磷酸酶的表达。这些结果表明，破骨细胞产生某些可溶性因子，刺激成骨细胞分化，他们被认为是与诱导更大的成骨细胞表面和骨体积的动物植入缺钙HA颗粒。(c)2009爱思唯尔有限公司保留所有权利。
Calcium-deficient hydroxyapatite (HA) granules with a unique spherical shape were prepared using an applied hydrothermal method. Spherical stoichiometric HA granules were also prepared by normal sintering and both granules were used for implantation into rat tibiae to compare the biological responses to each implant. Twelve and 24 weeks after implantation, the volume of calcium-deficient HA granules was significantly less than that of stoichiometric HA granules, and the biodegradability of calcium-deficient HA granules was confirmed. The larger number of osteoclasts, larger osteoblast surface and larger bone volume in the implanted area of calcium-deficient HA than those of stoichiometric HA suggested that osteoclastic resorption of calcium-deficient HA affected osteogenesis in that area. To analyze the direct contribution of osteoclasts to osteogenesis, C2C12 multipotent myoblastic cells, which have the potential to differentiate into osteoblasts in the presence of bone morphogenetic protein 2, were cultured with supernatants of osteoclasts cultured on calcium-deficient HA, stoichiometric HA, P-tricalcium phosphate disks or plastic dishes, or bone marrow macrophages cultured on plastic dishes. Supernatants of osteoclasts but not bone marrow macrophages stimulated the expression of Runx2 and osteocalcin in C2C12 cells in concert with bone morphogenetic protein 2. The expression of alkaline phosphatase was stimulated with supernatants of osteoclasts cultured on ceramic disks. These results suggested that osteoclasts produced certain soluble factors which stimulated osteoblastic differentiation and they were thought to be associated with the induction of a larger osteoblast surface and bone volume in the animals implanted with calcium-deficient HA granules. (c) 2009 Elsevier Ltd. All rights reserved.