Size-dependent cytotoxicity of amorphous silica nanoparticles in human hepatoma HepG2 cells
Size-dependent cytotoxicity of amorphous silica nanoparticles in human hepatoma HepG2 cells
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DOI：
10.1016/j.tiv.2011.05.003
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发表时间：
2011-10-01
影响因子：
3.2
通讯作者：
Sun, Zhiwei
中科院分区：
文献类型：
--
作者：
Li, Yang;Sun, Lei;Sun, Zhiwei
本研究的目的是比较不同大小的无定形二氧化硅颗粒对人体的潜在细胞毒性。研究了1种纳米二氧化硅(498 Nm)和3种纳米二氧化硅(68、43、19 nm)处理人肝癌细胞24 h后细胞形态、细胞存活率、细胞膜完整性、DNA损伤、细胞周期分布和细胞凋亡率的变化，结果表明，在人肝癌细胞中，二氧化硅颗粒的细胞毒作用强烈依赖于二氧化硅颗粒的大小，且小颗粒二氧化硅的毒性作用更强。为了进一步阐明细胞损伤的可能机制，测定了细胞内的活性氧自由基(ROS)。ROS水平也以大小依赖的方式被观察到。结果表明，微细颗粒对细胞内ROS水平无明显促进作用，但对细胞有损伤作用。因此，我们的数据表明，暴露于不同大小的二氧化硅颗粒导致了培养的HepG2细胞的大小依赖的细胞毒性，ROS的产生可能是一种可能的损伤途径，但可能不完全是二氧化硅颗粒所产生的毒性效应的原因。(C)2011爱思唯尔有限公司。保留所有权利。
The purpose of this study is to compare the potential cytotoxicity induced by amorphous silica particles with different sizes. The effects of one fine particle (498 nm) and three nanoparticles (68, 43, and 19 nm) on cultured human hepatoma (HepG2) cells were investigated by detecting morphological changes, cell viability, cytomembrane integrity, DNA damage, cell cycle distribution, and apoptosis after the cells were treated with 100 mu g/mL of four silica particles for 24 h. The results indicated that in HepG2 cells, the cytotoxicity generated by silica particles strongly depended on the particle size, and smaller silica particle possessed higher toxic effect. In order to further elucidate the possible mechanisms of cell injuries, intracellular reactive oxygen species (ROS) was measured. Increased ROS level was also observed in a size dependent way. However, the result showed the fine particle did not promote intracellular ROS level significantly, while cell injuries were detected in this treated group. Thus, our data demonstrated that exposure to different sizes of silica particles resulted in a size dependent cytotoxicity in cultured HepG2 cells, and ROS generation should be one possible damage pathway but might not be completely responsible for the toxic effect produced by silica particles. (C) 2011 Elsevier Ltd. All rights reserved.