[Low positive anti-HCV microparticle enzyme immunoassay results: do they predict hepatitis C virus infection?].
[Low positive anti-HCV microparticle enzyme immunoassay results: do they predict hepatitis C virus infection?].
复制标题
[低阳性抗HCV微粒酶联免疫分析结果：它们可以预测丙型肝炎病毒感染吗？]。
作者：
M. Sayan;M. Meriç;B. Mutlu;S. Celebi;A. Willke
丙型肝炎病毒（抗-HCV）抗体筛查测试可能会在土耳其等人群中产生假阳性结果，HCV感染率较低。这可能会导致不必要地使用昂贵的测试，如HCV RNA进行确认。然而，在S/Co（信号与截止值）比值较低的样本中，HCV RNA并不总是阳性。本回顾性研究旨在确定抗-HCV水平低的样本中HCV RNA阳性的频率。共对2002年6月至2005年11月期间通过微粒子酶免疫测定法（MEIA; v 3.0，AxSYM System，Abbott Laboratories，USA）检测抗-HCV阳性和通过实时聚合酶链反应（PCR; iCycler IQ，v 3.0a，Bio Rad Laboratory，USA）检测HCV RNA阳性的655例患者进行了评价。抗-HCV阳性368例（56%），HCV RNA未检出的样品中的S/Co比值低于3.8。因此，为了确认产生低和/或不确定灰色区抗HCV水平的样本，在使用HCV RNA检测之前，免疫印迹方法、用另一种酶免疫测定法重新检测相同样本或检测新样本将是适当和经济的。
Antibody screening tests for hepatitis C virus (anti-HCV) can yield false positive results among populations such as Turkey, with a low prevalence of HCV infection. This may lead to the unnecessary use of expensive tests like HCV RNA for confirmation. However, HCV RNA is not always positive in samples with low S/Co (signal to cutoff) ratios. This retrospective study was conducted to determine the frequency of HCV RNA positivity in the samples with low anti-HCV levels. A total of 655 patients who were positive for anti-HCV by microparticle enzyme immunoassay (MEIA; v 3.0, AxSYM System, Abbott Laboratories, USA) and positive for HCV RNA by real-time polymerase chain reaction (PCR; iCycler IQ, v 3.0a, Bio Rad Laboratory, USA) between June, 2002 and November, 2005 were evaluated. Anti-HCV positivity was found in 368 (56%) patients and HCV RNA was not detected in samples with S/Co ratios lower than 3.8. As a result, for the confirmation of the samples yielding low and/or indeterminate grey zone anti-HCV levels, immunoblot methods, retesting of the same sample with another enzyme immunassay or testing of a new sample, would be appropriate and economical prior to use of HCV RNA tests.