Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds
Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds
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DOI：
10.1016/j.biortech.2007.07.005
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发表时间：
2008-05-01
影响因子：
11.4
通讯作者：
Bhaskar, N.
中科院分区：
文献类型：
--
作者：
Ganesan, P.;Kumar, Chandini S.;Bhaskar, N.
对三种印度红藻，即龙须菜、江豚和刺五加的体外抗氧化活性进行了研究。测定了粗甲醇抽提物的总酚含量和还原能力。考察了总甲醇提取物和五种不同溶剂(石油醚、乙酸乙酯、二氯甲烷、丁醇和水)的抗氧化活性。在所有提取物中，辣根提取物具有较高的总抗氧化活性(32.01 mg抗坏血酸当量/g提取物)。没食子酸当量为16.26 mg没食子酸当量/g提取物中的酚类物质含量较高。粗甲醇抽提物的还原能力随抽提物浓度的增加而增大。与标准抗氧化剂(α-生育酚)相比，龙须草具有更强的还原能力和清除羟基自由基活性。所有海藻的总酚含量差异显著(P<；0.05)。三种海藻甲醇提取物的体外抗氧化活性均呈剂量依赖关系，且随提取物浓度的增加而增强。(C)2007爱思唯尔有限公司。保留所有权利。
In vitro antioxidant activities of three selected Indian red seaweeds - viz., Euchema kappaphycus, Gracilaria edulis and Acanthophora spicifera were evaluated. Total phenolic content and reducing power of crude methanol extract were determined. The antioxidant activities of total methanol extract and five different solvent fractions (viz., petroleum ether (PE), ethyl acetate (EA), dichloromethane (DCM), butanol (BuOH) and aqueous) were also evaluated. EA fraction of A. spicifera exhibited higher total antioxidant activity (32.01 mg ascorbic acid equivalent/g extract) among all the fractions. Higher phenolic content (16.26 mg gallic acid equivalent/g extract) was noticed in PE fraction of G. edulis. Reducing power of crude methanol extract increased with increasing concentration of the extract. Reducing power and hydroxyl radical scavenging activity of E. kappaphycus were higher compared to standard antioxidant (alpha-tocopherol). The total phenol content of all the seaweeds was significantly different (P < 0.05). In vitro antioxidant activities of methanol extracts of all the three seaweeds exhibited dose dependency; and increased with increasing concentration of the extract. (c) 2007 Elsevier Ltd. All rights reserved.