The reverse transcriptase.
The reverse transcriptase.
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逆转录酶。
DOI：
10.1016/0304-419x(77)90005-1
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
I. Verma
中科院分区：
文献类型：
--
作者：
I. Verma
1970年夏天，Baltimore[1]、Temin和Mizutani[1]报道了RNA肿瘤病毒的病毒粒子中存在一种DNA聚合酶。病毒RNA作为模板来指导脱氧核糖核苷三磷酸的结合。这一发现为RNA肿瘤病毒通过DNA中间体进行复制的观点提供了可信的支持[3,4]。自发现以来，这种酶一直是许多研究人员非常感兴趣的对象，并且已经积累了大量关于其性质，功能和结构的数据。该酶在文献中也被称为RNA依赖DNA聚合酶、RNA稀释DNA聚合酶和RNA肿瘤病毒的DNA聚合酶（EC 2.7）。7.7)。在这篇文章中，我将用它更流行的名字——逆转录酶来指代它。最近有几篇关于逆转录酶的优秀综述文章（一般介绍，Temin and Baltimore [5], Temin [6,7], Tooze[8]；详细文献综述，Sarin and Gallo [9], Green and Gerard [10]， Wu and Gallo[11]和Sarngadharan et al.[12]）。在这篇文章中，我将局限于讨论逆转录酶的结构，它在RNA肿瘤病毒生命周期中的作用以及对分子生物学的更广泛的影响。Taylor， J.和Weinberg， RA的文章详细描述了逆转录酶合成DNA的机制和性质。
In the summer of 1970, Baltimore [1] and Temin and Mizutani [2] reported the presence of a DNA polymerase in the virions of RNA tumor viruses. The viral RNA acted as the template to direct the incorporation of deoxyribonucleoside triphosphates. This finding furnished credible support to the notion that RNA tumor viruses replicate via a DNA intermediate [3, 4]. Since its discovery, this enzyme has been an object of great interest to many investigators and a formidable amount of data has been accumulated on its propelties, function and structure. The enzyme has been alternatively referred to in literature as RNA-dependent DNA polymerase, RNA-dilected DNA polymerase and DNA polymerase of RNA tumor viruses (EC 2.7. 7.7). In this article, I will refer to it by its more popular name, reverse transcriptase. Several outstanding review articles have recently been written on reverse transcriptase (for genelal introduction, Temin and Baltimore [5], Temin [6, 7], Tooze [8]; for detailed literature review Sarin and Gallo [9], Green and Gerard [10], Wu and Gallo [11] and Sarngadharan et al.[12]). In this article, I shall confine myself to the discussion of the structure of reverse transcriptase, its role in the life cycle of RNA tumor viruses and broader implications to molecular biology. Accompanying articles by Taylor, J. and Weinberg, RA describe in detail the mechanism and the nature of DNA synthesized by reverse transcriptase.