Role of material surfaces in regulating bone and cartilage cell response
Role of material surfaces in regulating bone and cartilage cell response
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DOI：
10.1016/0142-9612(96)85758-9
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发表时间：
1996-01-01
期刊：
影响因子：
14
通讯作者：
Schwartz, Z
中科院分区：
文献类型：
--
作者：
Boyan, BD;Hummert, TW;Schwartz, Z
体外和体内的组织工程涉及细胞与材料表面的相互作用。表面的性质可以直接影响细胞反应，最终影响新组织形成的速度和质量。表面的初始事件包括从周围流体定向吸附分子，产生细胞响应的条件界面。总体形态，以及表面的微观形貌和化学性质，决定了哪些分子可以吸附以及细胞如何附着和排列。细胞与其基质的局部附着决定了细胞形状，当通过细胞骨架转导到细胞核时，导致特定表型的表达。成骨细胞和软骨细胞对表面粗糙度和表面化学性质的细微差异敏感。比较软骨细胞对不同堆积的TiO 2的反应的研究表明，细胞也可以在该水平上区分表面。细胞反应还取决于局部环境和反应细胞的成熟状态。优化特定位点组织工程的表面结构是一种选择;用生物制剂修饰表面是另一种选择。
Tissue engineering in vitro and in vivo involves the interaction of cells with a material surface. The nature of the surface can directly influence cellular response, ultimately affecting the rate and quality of new tissue formation. Initial events at the surface include the orientated adsorption of molecules from the surrounding fluid, creating a conditioned interface to which the cell responds. The gross morphology, as well as the microtopography and chemistry of the surface, determine which molecules can adsorb and how cells will attach and align themselves. The focal attachments made by the cells with their substrate determine cell shape which, when transduced via the cytoskeleton to the nucleus, result in expression of specific phenotypes. Osteoblasts and chondrocytes are sensitive to subtle differences in surface roughness and surface chemistry. Studies comparing chondrocyte response to TiO2 of differing crystallinities show that cells can discriminate between surfaces at this level as well. Cellular response also depends on the local environmental and state of maturation of the responding cells. Optimizing surface structure for site-specific tissue engineering is one option; modifying surfaces with biologicals is another.