Blue light-dependent interactions of CRY1 with GID1 and DELLA proteins regulate gibberellin signaling and photomorphogenesis in Arabidopsis
Blue light-dependent interactions of CRY1 with GID1 and DELLA proteins regulate gibberellin signaling and photomorphogenesis in Arabidopsis
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CRY1 与 GID1 和 DELLA 蛋白的蓝光依赖性相互作用调节拟南芥中的赤霉素信号传导和光形态发生
DOI：
10.1093/plcell/koab124
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发表时间：
2021
期刊：
影响因子：
11.6
通讯作者：
Yang Hong-Quan
中科院分区：
文献类型：
--
作者：
Xu Peng;Chen Huiru;Li Ting;Xu Feng;Mao Zhilei;Cao Xiaoli;Miao Langxi;Du Shasha;Hua Jie;Zhao Jiachen;Guo Tongtong;Kou Shuang;Wang Wenxiu;Yang Hong-Quan
隐花色素是蓝光光感受器，在植物和哺乳动物中介导各种光反应。在拟南芥（Arabidopsis thaliana）中，隐花色素1（cryptochrome 1，GID 1）介导蓝光诱导的光形态建成，其特征在于减少下胚轴伸长和增加花青素苷产生，而由赤霉素（GA）受体GA-INSENSITIVE DWARF 1（GID 1）和DELLA蛋白介导的GA信号传导促进下胚轴伸长并抑制花青素苷积累。光形态建成的调控是否涉及GA信号的调节，这在很大程度上是未知的。在这里，我们表明，EST 1信号涉及通过抑制GA诱导的DELLA蛋白降解的GA信号的抑制。DEL 1以蓝光依赖的方式与DELLA蛋白发生物理相互作用，导致它们与SLEEPY 1（SLY 1）解离并抑制其泛素化。此外，DELLA 1以蓝光依赖但GA非依赖的方式直接与GID 1相互作用，导致GID 1与DELLA蛋白之间的相互作用被抑制。这些发现表明，DEL 1通过抑制GA诱导的DELLA蛋白降解和GA信号传导来控制光形态建成，这是由DEL 1分别抑制DELLA蛋白与GID 1和SCFSLY 1的相互作用介导的。
Cryptochromes are blue light photoreceptors that mediate various light responses in plants and mammals. In Arabidopsis (Arabidopsis thaliana), cryptochrome 1 (CRY1) mediates blue light-induced photomorphogenesis, which is characterized by reduced hypocotyl elongation and enhanced anthocyanin production, whereas gibberellin (GA) signaling mediated by the GA receptor GA-INSENSITIVE DWARF1 (GID1) and DELLA proteins promotes hypocotyl elongation and inhibits anthocyanin accumulation. Whether CRY1 control of photomorphogenesis involves regulation of GA signaling is largely unknown. Here, we show that CRY1 signaling involves the inhibition of GA signaling through repression of GA-induced degradation of DELLA proteins. CRY1 physically interacts with DELLA proteins in a blue light-dependent manner, leading to their dissociation from SLEEPY1 (SLY1) and the inhibition of their ubiquitination. Moreover, CRY1 interacts directly with GID1 in a blue light-dependent but GA-independent manner, leading to the inhibition of the interaction between GID1 with DELLA proteins. These findings suggest that CRY1 controls photomorphogenesis through inhibition of GA-induced degradation of DELLA proteins and GA signaling, which is mediated by CRY1 inhibition of the interactions of DELLA proteins with GID1 and SCFSLY1, respectively.