Cytokines in the oral mucosa of mice infected with Candida albicans
Cytokines in the oral mucosa of mice infected with Candida albicans
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DOI：
10.1034/j.1399-302x.2002.170607.x
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发表时间：
2002-12-01
影响因子：
--
通讯作者：
Ashman, RB
中科院分区：
文献类型：
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作者：
Farah, CS;Gotjamanos, T;Ashman, RB
口咽念珠菌病与细胞介导的免疫缺陷有关，常见于免疫功能低下的患者。我们之前已经证明，T 细胞缺陷的 BALB/c 裸鼠 (nu/nu) 对口咽念珠菌病极其敏感，慢性感染的恢复依赖于 CD4 T 淋巴细胞。在这项研究中，我们描述了淋巴细胞重建的免疫缺陷小鼠及其正常小鼠的局部组织细胞因子谱。小鼠经口感染10(8)个白色念珠菌细胞，并在第0、4、8和14天采集口腔组织样本。在口腔接种后用3 x 10(7)个幼稚淋巴细胞对裸鼠进行重建。在从口腔感染中恢复的受感染的正常小鼠以及初始对照组的口腔组织中发现了白细胞介素（IL）-6、干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α。第 4 天和第 8 天在裸口腔组织中鉴定出 TNF-α，但仅在淋巴细胞重建后才进行。在整个实验的任何时间点，在胸腺正常或无胸腺的小鼠中均未检测到IL-2、IL-4或IL-10。这项研究证实了重组裸鼠中 T 淋巴细胞的功能活性，并表明 TNF-α 可能是口咽念珠菌病恢复的重要介质。
Oropharyngeal candidiasis is associated with defects in cell-mediated immunity, and is commonly seen in immunocompromised patients. We have previously shown that T-cell-deficient BALB/c nude (nu/nu) mice are extremely susceptible to oropharyngeal candidiasis, and that recovery from a chronic infection is dependent on CD4 T lymphocytes. In this study we describe the local tissue cytokine profile in lymphocyte-reconstituted immunodeficient mice and their euthymic counterparts. Mice were infected orally with 10(8) cells of the yeast Candida albicans , and oral tissues sampled on days 0, 4, 8, and 14. Nude mice were reconstituted with 3 x 10(7) naive lymphocytes following oral inoculation. Interleukin (IL)-6, interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha were identified in the oral tissues of infected euthymic mice recovering from oral infection, as well as naive controls. TNF-alpha was identified in nude oral tissue on days 4 and 8, but only after lymphocyte reconstitution. No IL-2, IL-4 or IL-10 was detected in either euthymic or athymic mice at any time-point throughout the experiment. This study confirms the functional activity of T lymphocytes in reconstituted nude mice, and suggests that TNF-alpha may be an important mediator in the recovery from oropharyngeal candidiasis.