Differential Synaptic Dynamics and Circuit Connectivity of Hippocampal and Thalamic Inputs to the Prefrontal Cortex.
Differential Synaptic Dynamics and Circuit Connectivity of Hippocampal and Thalamic Inputs to the Prefrontal Cortex.
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DOI：
10.1093/texcom/tgaa084
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发表时间：
2020
影响因子：
--
通讯作者：
Kellendonk C
中科院分区：
文献类型：
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作者：
Canetta S;Teboul E;Holt E;Bolkan SS;Padilla-Coreano N;Gordon JA;Harrison NL;Kellendonk C
内侧前额叶皮质(MPFC)整合来自多个皮质下区域的信息，包括丘脑内侧背核(MD)和腹侧海马区(VHPC)。MPFC如何以不同的方式处理这些输入尚不清楚。一种可能性是，这两种输入针对的是谨慎的mPFC细胞群体。或者，单个前额叶细胞可以接受汇聚的输入，但根据突触差异，如交流频率，区分两种输入。为了解决这一问题，我们利用双波长光遗传学方法将MD和vHPC的输入刺激到单一的、基因定义的mPFC神经元亚型上。具体地说，我们比较了两种输入到mPFC锥体细胞的汇聚和突触动力学，以及表达小白蛋白(PV)和血管活性肠肽(VIP)的中间神经元。我们发现，mPFC第2/3层的所有锥体神经元都接受MD和vHPC的汇聚输入。相反，PV神经元从MD接收偏向输入，而VIP细胞从vHPC接收偏向输入。与目标无关，MD输入在更高的频率(20赫兹)下比vHPC输入更可靠地传输信息。因此，MD和vHPC投射在功能上会聚到mPFC锥体细胞，但这两种输入都有频率相关的突触动力学和离散的中间神经元群体的优先参与。
The medial prefrontal cortex (mPFC) integrates inputs from multiple subcortical regions including the mediodorsal nucleus of the thalamus (MD) and the ventral hippocampus (vHPC). How the mPFC differentially processes these inputs is not known. One possibility is that these two inputs target discreet populations of mPFC cells. Alternatively, individual prefrontal cells could receive convergent inputs but distinguish between both inputs based on synaptic differences, such as communication frequency. To address this, we utilized a dual wavelength optogenetic approach to stimulate MD and vHPC inputs onto single, genetically defined mPFC neuronal subtypes. Specifically, we compared the convergence and synaptic dynamics of both inputs onto mPFC pyramidal cells, and parvalbumin (PV)- and vasoactive intestinal peptide (VIP)-expressing interneurons. We found that all individual pyramidal neurons in layer 2/3 of the mPFC receive convergent input from both MD and vHPC. In contrast, PV neurons receive input biased from the MD, while VIP cells receive input biased from the vHPC. Independent of the target, MD inputs transferred information more reliably at higher frequencies (20 Hz) than vHPC inputs. Thus, MD and vHPC projections converge functionally onto mPFC pyramidal cells, but both inputs are distinguished by frequency-dependent synaptic dynamics and preferential engagement of discreet interneuron populations.