Patterned co-culture of primary hepatocytes and fibroblasts using polyelectrolyte multilayer templates
Patterned co-culture of primary hepatocytes and fibroblasts using polyelectrolyte multilayer templates
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DOI：
10.1002/mabi.200600205
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发表时间：
2007-03-08
影响因子：
4.6
通讯作者：
Chan, Christina
中科院分区：
文献类型：
--
作者：
Kidambi, Srivatsan;Sheng, Lufang;Chan, Christina
本文描述了使用合成离子聚合物的逐层沉积而不借助于粘附蛋白/配体（如胶原蛋白或纤连蛋白）形成图案化细胞共培养物。在这项研究中，我们使用合成的聚合物，即聚（二烯丙基二甲基氯化铵）（PDAC）和磺化聚苯乙烯（SPS）作为聚阳离子和聚阴离子，分别构建多层膜。我们通过微接触印刷PDAC到SPS表面上或反之亦然，在多层膜（PEM）表面上形成SPS图案。为了在PEM上创建图案化的共培养物，我们利用了原代肝细胞在SPS上的优先附着和扩散，而不是PDAC表面。相比之下，成纤维细胞容易附着到PDAC和SPS表面，因此，我们能够在合成PEM表面上获得成纤维细胞和原代肝细胞的图案化共培养物。我们用显微镜和生化分析表征了图案化细胞共培养物的形态和肝脏特异性功能。我们的研究结果表明，一种替代的方法来制造特定的细胞-细胞和细胞-表面相互作用的控制共培养物，这种方法提供了灵活性，在设计细胞特异性表面的组织工程应用。
This paper describes the formation of patterned cell co-cultures using the layer-by-layer deposition of synthetic ionic polymers and without the aid of adhesive proteins/ligands such as Collagen or fibronectin. In this study, we used synthetic polymers, namely poly(diallyldi-methylammoniurn chloride) (PDAC) and sulfonated polystyrene (SPS) as the polycation and polyanion, respectively, to build the multilayer films. We formed SPS patterns on polyelectrolyte multilayer (PEM) surfaces either by microcontact printing PDAC onto SPS surfaces or vice-versa. To create patterned co-cultures on PEMs, we capitalize on the preferential attachment and spreading of primary hepatocytes on SPS as opposed to PDAC surfaces. In contrast, fibroblasts readily attached to both PDAC and SPS surfaces, and as a result, we were able to obtain patterned co-cultures of fibroblast and primary hepatocytes on synthetic PEM surfaces. We characterized the morphology and hepatic-specific functions of the patterned cell co-cultures with microscopy and biochemical assays. Our results suggest an alternative approach to fabricating controlled co-cultures with specified cell-cell and cell-surface interactions; this approach provides flexibility in designing cell-specific surfaces for tissue engineering applications.