The complete genome sequencing of Prevotella intermedia strain OMA14 and a subsequent fine-scale, intra-species genomic comparison reveal an unusual amplification of conjugative and mobile transposons and identify a novel Prevotella-lineage-specific repeat.
The complete genome sequencing of Prevotella intermedia strain OMA14 and a subsequent fine-scale, intra-species genomic comparison reveal an unusual amplification of conjugative and mobile transposons and identify a novel Prevotella-lineage-specific repeat.
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DOI：
10.1093/dnares/dsv032
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Nakayama K
中科院分区：
文献类型：
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作者：
Naito M;Ogura Y;Itoh T;Shoji M;Okamoto M;Hayashi T;Nakayama K
中间普雷沃氏菌是一种与牙周病有关的致病菌。在这里，我们展示了这种细菌的临床菌株OMA14的完整基因组序列，以及与同一物种的菌株17的比较基因组分析结果，菌株17的基因组也已测序，但尚未完全分析。这两种菌株的基因组都由两条圆形染色体组成：较大的染色体大小相似，并表现出较高的基因组织整体线性，而较小的染色体则表现出显著的大小差异，并经历了显著的基因组重排。Pre的独特功能。中间基因组是指在第二染色体上存在大量必需基因和丰富的共轭和可移动转座子（ctn和mtn）。ctn / mtn在第二条染色体中特别丰富，参与其广泛的基因组重排，并将许多菌株特异性基因引入每个菌株。我们还发现了一个新的188bp重复序列，在Pre中被高度扩增。中间媒介，并专门分布在Pre中。intermedia-related物种。这些发现扩大了我们对Pre基因特征的理解。中间介质以及ctn和mtn在细菌进化中的作用。
Prevotella intermedia is a pathogenic bacterium involved in periodontal diseases. Here, we present the complete genome sequence of a clinical strain, OMA14, of this bacterium along with the results of comparative genome analysis with strain 17 of the same species whose genome has also been sequenced, but not fully analysed yet. The genomes of both strains consist of two circular chromosomes: the larger chromosomes are similar in size and exhibit a high overall linearity of gene organizations, whereas the smaller chromosomes show a significant size variation and have undergone remarkable genome rearrangements. Unique features of the Pre. intermedia genomes are the presence of a remarkable number of essential genes on the second chromosomes and the abundance of conjugative and mobilizable transposons (CTns and MTns). The CTns/MTns are particularly abundant in the second chromosomes, involved in its extensive genome rearrangement, and have introduced a number of strain-specific genes into each strain. We also found a novel 188-bp repeat sequence that has been highly amplified in Pre. intermedia and are specifically distributed among the Pre. intermedia-related species. These findings expand our understanding of the genetic features of Pre. intermedia and the roles of CTns and MTns in the evolution of bacteria.