Evaluation of rhamnolipid and surfactin to reduce the adhesion and remove biofilms of individual and mixed cultures of food pathogenic bacteria
Evaluation of rhamnolipid and surfactin to reduce the adhesion and remove biofilms of individual and mixed cultures of food pathogenic bacteria
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DOI：
10.1016/j.foodcont.2011.11.025
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发表时间：
2012-06-01
期刊：
影响因子：
6
通讯作者：
Nitschke, Marcia
中科院分区：
文献类型：
--
作者：
do Valle Gomes, Milene Zezzi;Nitschke, Marcia
生物膜引起了食品工业的高度关注，因为它们可能是导致食品腐败和疾病传播的持续污染源。为了避免细菌粘附和生物膜的形成，另一种方法是使用生物表面活性剂和微生物化合物对表面进行预处理，这些微生物化合物可以改变表面的物理化学性质，从而改变细菌相互作用，从而改变粘附力。评估了不同浓度的生物表面活性剂（来自枯草芽孢杆菌的表面活性剂和来自铜绿假单胞菌的鼠李糖脂）以减少粘附并破坏食源性致病菌的生物膜。使用聚苯乙烯作为模型表面，研究了金黄色葡萄球菌、单核细胞增生李斯特菌和肠炎沙门氏菌的单独培养物和混合培养物。 0.25%表面活性剂预处理可将单核细胞增生李斯特菌和肠炎沙门氏菌的粘附力降低42.0%，而1.0%鼠李糖脂处理可将单核细胞增生李斯特菌和金黄色葡萄球菌与聚苯乙烯的粘附力降低57.8%和67.8%。生物表面活性剂在避免混合培养物的粘附方面效果较差。 与单个培养物相比，细菌。与0.1%浓度的surfactin接触2小时后，预先形成的金黄色葡萄球菌生物膜减少了63.7%，单增李斯特菌减少了95.9%，肠炎沙门氏菌减少了35.5%，混合培养物生物膜减少了58.5%。 0.25%浓度的鼠李糖脂接触2小时后，去除了58.5%的金黄色葡萄球菌生物膜、26.5%的单增李斯特菌、23.0%的肠炎沙门氏菌和24.0%的混合培养物。一般来说，生物表面活性剂浓度和接触时间的增加会降低生物膜去除率。这些结果表明，可以探索表面活性素和鼠李糖脂来控制食源性病原体的附着和破坏单个和混合培养物的生物膜。 (C) 2011 Elsevier Ltd. 保留所有权利。
Biofilms represent a great concern for food industry, since they can be a source of persistent contamination leading to food spoilage and to the transmission of diseases. To avoid the adhesion of bacteria and the formation of biofilms, an alternative is the pre-conditioning of surfaces using biosurfactants, microbial compounds that can modify the physicochemical properties of surfaces changing bacterial interactions and consequently adhesion. Different concentrations of the biosurfactants, surfactin from Bacillus subtilis and rhamnolipids from Pseudomonas aeruginosa, were evaluated to reduce the adhesion and to disrupt biofilms of food-borne pathogenic bacteria. Individual cultures and mixed cultures of Staphylococcus aureus, Listeria monocytogenes and Salmonella Enteritidis were studied using polystyrene as the model surface. The pre-conditioning with surfactin 0.25% reduced by 42.0% the adhesion of L monocytogenes and S. Enteritidis, whereas the treatment using rhamnolipids 1.0% reduced by 57.8% adhesion of L monocytogenes and by 67.8% adhesion of S. aureus to polystyrene.Biosurfactants were less effective to avoid adhesion of mixed cultures of the bacteria when compared with individual cultures. After 2 h contact with surfactin at 0.1% concentration, the pre-formed biofilms of S. aureus were reduced by 63.7%, L. monocytogenesby 95.9%, S. Enteritidis by 35.5% and the mixed culture biofilm by 58.5%. The rhamnolipids at 0.25% concentration removed 58.5% the biofilm of S. aureus, 26.5% of L monocytogenes, 23.0% of S. Enteritidis and 24.0% the mixed culture after 2 h contact. In general, the increase in concentration of biosurfactants and in the time of contact decreased biofilm removal percentage. These results suggest that surfactin and rhamnolipids can be explored to control the attachment and to disrupt biofilms of individual and mixed cultures of the food-borne pathogens. (C) 2011 Elsevier Ltd. All rights reserved.