The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea
The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea
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DOI：
10.1007/s10529-010-0497-0
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发表时间：
2011-04-01
影响因子：
2.7
通讯作者：
Yoshida, Makoto
中科院分区：
文献类型：
--
作者：
Hashimoto, Kohsuke;Yokota, Etsuo;Yoshida, Makoto
灰盖鬼伞（Coprinopsiscinerea）是一种植物性嗜盐担子菌，在降解纤维素的过程中产生并分泌多种纤维素酶，作为其主要的胞外蛋白。虽然这种纤维素酶的酶学特性已被频繁报道，但其分泌机制仍不清楚。本研究的重点是肌球蛋白，肌动蛋白为基础的运动蛋白，参与蛋白质分泌的C。灰叶在纤维素酶诱导培养过程中，用肌球蛋白ATP酶的抑制剂2，3-丁二酮2-单肟（BDM）处理菌丝体时，没有观察到纤维素酶活性。此外，BDM治疗中断定位的高尔基体，但不是内质网。从C. cinerea genome database.在纤维素酶诱导的条件下生长，这些基因的转录得到促进。
The plant-saprophytic basidiomycete, Coprinopsis cinerea, produces and secretes various cellulases during cellulose degradation as the main extracellular proteins. Although enzymatic characterization of such cellulases has been frequently reported, the mechanism of their secretion remains unclear. This study focused on myosins, actin-based motor proteins, involved in protein secretion in C. cinerea. During cultivation under cellulase-inducing condition, no cellulase activity was observed when the mycelia were treated with 2,3-butanedione 2-monoxime (BDM), a general inhibitor of myosin ATPase. Furthermore, BDM treatment disrupted the localization of the Golgi apparatus, but not that of the endoplasmic reticulum. Three genes encoding myosin-like proteins (CcMyo1, CcMyo2 and CcMyo5) were identified from the C. cinerea genome database. Transcription of these genes was promoted when the fungus was grown under cellulase-inducing condition.