Automated cell lineage construction - A rapid method to analyze clonal development established with murine neural progenitor cells
Automated cell lineage construction - A rapid method to analyze clonal development established with murine neural progenitor cells
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DOI：
10.4161/cc.5.3.2426
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发表时间：
2006-02-01
期刊：
影响因子：
4.3
通讯作者：
Roysam, B
中科院分区：
文献类型：
--
作者：
Al-Kofahi, O;Radke, RJ;Roysam, B
了解细胞谱系关系是了解发育的基础，并且可以阐明疾病的病因和进展。我们提出了一种从图像序列中自动跟踪增殖、迁移细胞谱系的方法。该方法适用于体外或体内收集的图像序列。目前，随着时间的推移从祖细胞生成谱系树是一个繁琐的手动过程，这限制了可以实际分析的细胞测量的数量。相比之下，自动化方法快速且易于应用，并产生大量测量结果，包括每帧中每个细胞的精确位置、形状、细胞间接触、运动性和祖先，以及关键事件的准确计时，例如。例如，有丝分裂和细胞死亡。此外，它还会自动生成可立即访问的图形输出。将其应用于细胞培养中生长的小鼠神经祖细胞的克隆发育，揭示了神经元和神经胶质生产过程中细胞周期速率的复杂变化。该方法能够对细胞行为随时间的变化进行一定程度的定量分析，这在以前是不可行的。
Understanding cell lineage relationships is fundamental to understanding development, and can shed light on disease etiology and progression. We present a method for automated tracking of lineages of proliferative, migrating cells from a sequence of images. The method is applicable to image sequences gathered either in vitro or in vivo. Currently, generating lineage trees from progenitor cells over time is a tedious, manual process, which limits the number of cell measurements that can be practically analyzed. In contrast, the automated method is rapid and easily applied, and produces a wealth of measurements including the precise position, shape, cell-cell contacts, motility and ancestry of each cell in every frame, and accurate timings of critical events, e. g., mitosis and cell death. Furthermore, it automatically produces graphical output that is immediately accessible. Application to clonal development of mouse neural progenitor cells growing in cell culture reveals complex changes in cell cycle rates during neuron and glial production. The method enables a level of quantitative analysis of cell behavior over time that was previously infeasible.