Delivery strategies to control inflammatory response: Modulating M1-M2 polarization in tissue engineering applications.
Delivery strategies to control inflammatory response: Modulating M1-M2 polarization in tissue engineering applications.
复制标题
控制炎症反应的输送策略：调节组织工程应用中的M1-M2极化。
DOI：
10.1016/j.jconrel.2016.01.026
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发表时间：
2016-10-28
期刊：
影响因子：
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通讯作者：
Khademhosseini A
中科院分区：
文献类型：
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作者：
Alvarez MM;Liu JC;Trujillo-de Santiago G;Cha BH;Vishwakarma A;Ghaemmaghami AM;Khademhosseini A
巨噬细胞是许多生理场景中的关键角色，包括组织动态平衡。在对损伤的反应中，巨噬细胞亚群之间的平衡通常从M1表型(促炎)转变为M2表型(抗炎)。在组织工程学的情况下，在植入任何装置后，都希望对M1-M2的进展进行控制，并确保从炎症到愈合阶段的及时和平稳过渡。在这篇综述中，我们简要介绍关于巨噬细胞功能和命名的知识现状。接下来，我们讨论在组织工程应用的背景下，使用控制释放策略来调节M1和M2表型之间的平衡。我们讨论了与抗炎分子(包括核酸)的释放和细胞因子的顺序释放有关的最新文献，以促进M1-M2的及时转移。此外，我们描述了在支架提供的物理和/或机械提示的刺激下，巨噬细胞作为控释剂的使用。此外，我们还讨论了“智能”可植入支架的当前和未来应用，这些支架能够控制与愈合和血管形成相关的一连串生化事件。最后，我们对目前面临的挑战和未来的研究方向提出了自己的看法，以提高我们对M1-M2巨噬细胞平衡的理解，并在组织工程和再生医学应用中适当地利用它。
Macrophages are key players in many physiological scenarios including tissue homeostasis. In response to injury, typically the balance between macrophage sub-populations shifts from an M1 phenotype (pro-inflammatory) to an M2 phenotype (anti-inflammatory). In tissue engineering scenarios, after implantation of any device, it is desirable to exercise control on this M1-M2 progression and to ensure a timely and smooth transition from the inflammatory to the healing stage. In this review, we briefly introduce the current state of knowledge regarding macrophage function and nomenclature. Next, we discuss the use of controlled release strategies to tune the balance between the M1 and M2 phenotypes in the context of tissue engineering applications. We discuss recent literature related to the release of anti-inflammatory molecules (including nucleic acids) and the sequential release of cytokines to promote a timely M1-M2 shift. In addition, we describe the use of macrophages as controlled release agents upon stimulation by physical and/or mechanical cues provided by scaffolds. Moreover, we discuss current and future applications of “smart” implantable scaffolds capable of controlling the cascade of biochemical events related to healing and vascularization. Finally, we provide our opinion on the current challenges and the future research directions to improve our understanding of the M1-M2 macrophage balance and properly exploit it in tissue engineering and regenerative medicine applications.