The nuclear receptor hepatocyte nuclear factor 4α acts as a morphogen to induce the formation of microvilli
The nuclear receptor hepatocyte nuclear factor 4α acts as a morphogen to induce the formation of microvilli
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DOI：
10.1083/jcb.200608012
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发表时间：
2006-12-18
影响因子：
7.8
通讯作者：
Sawada, Norimasa
中科院分区：
文献类型：
--
作者：
Chiba, Hideki;Sakai, Naoyuki;Sawada, Norimasa
微绒毛是在顶端质膜上发现的基于肌动蛋白的细胞器，其参与营养吸收和信号转导。许多组件，包括ezrin/radixin/moesin（ERM）蛋白，已被确定连接丝状肌动蛋白跨膜蛋白，但驱动微绒毛生物发生的信号是未知的。在这项研究中，我们表明，条件和/或异位表达的核受体，肝细胞核因子4 α（HNF 4 α），触发微绒毛形态发生。我们还表明，HNF 4 α的表达诱导ERM结合磷蛋白50（EBP 50）的表达和衰减EBP 50使用RNA干扰抑制微绒毛的发展。我们的结论是，HNF 4 α作为一种形态发生素触发微绒毛的形成。
Microvilli are actin-based organelles found on apical plasma membranes that are involved in nutrient uptake and signal transduction. Numerous components, including ezrin/radixin/moesin (ERM) proteins, have been identified that link filamentous actins to transmembrane proteins, but the signals driving microvillus biogenesis are not known. In this study, we show that the conditional and/or ectopic expression of a nuclear receptor, hepatocyte nuclear factor 4 alpha (HNF4 alpha), triggers microvillus morphogenesis. We also demonstrate that HNF4 alpha expression induces ERM-binding phosphoprotein 50 (EBP50) expression and that attenuation of EBP50 using RNA interference inhibits microvillus development. We conclude that HNF4 alpha acts as a morphogen to trigger microvillus formation.