Duplication and distinct expression patterns of two thrombospondin-1 isoforms in teleost fishes
Duplication and distinct expression patterns of two thrombospondin-1 isoforms in teleost fishes
复制标题
硬骨鱼中两种血小板反应蛋白-1亚型的重复和不同表达模式
DOI：
10.1016/j.gep.2009.05.001
复制
发表时间：
2009-09-01
影响因子：
1.2
通讯作者：
Wang, De-Shou
中科院分区：
文献类型：
--
作者：
Wu, Feng-Rui;Zhou, Lin-Yan;Wang, De-Shou
Two types of thrombospondin-1 (named TSP-1a and TSP-1b) were cloned from two species of teleosts, the Nile tilapia and medaka. Phylogenetic analysis of these TSP-1 sequences, together with those available from other vertebrates further demonstrated that two types of TSP-1 exist only in teleosts, extending the finding in fugu and tetraodon to two additional fish species. The expression of both genes was examined using tilapia at various developmental stages. Tilapia TSP-1a and TSP-1b were each expressed in a wide range of tissues examined. The early expression of TSP-1b in both XX and XY gonads from 5 dah (day after hatching) onwards suggested an important role in the formation of gonads, while the expression of TSP-1a only in ovaries during later stages of development (from 120 dah onwards) may suggest that TSP-1a is involved in oogenesis. During the 14-day spawning cycle, the expression of both types of TSP-1 exhibited distinct peaks at day 5 (peak of vitellogenesis) and day 12 (oocyte maturation). In situ hybridization analyses revealed differential expression, with TSP-1a occurring in granulosa cells and TSP- 1b in theca cells. Furthermore, both TSP-1a and -1b were expressed in skeletal tissues but with clear temporal and spatial differences. In contrast, only TSP-1b was found in the myosepta. The positive signals of both TSP-1a and TSP-1b were also detected in the heart and spleen, and TSP-1a in brain and intestine by both RT-PCR and in situ hybridization. (C) 2009 Elsevier B.V. All rights reserved.
