Use of molecular methods to estimate the size and distribution of mycelial individuals of the ectomycorrhizal basidiomycete Pisolithus tinctorius
Use of molecular methods to estimate the size and distribution of mycelial individuals of the ectomycorrhizal basidiomycete Pisolithus tinctorius
复制标题
DOI：
10.1017/s0953756297004954
复制
发表时间：
1998-03-01
影响因子：
--
通讯作者：
Cairney, JWG
中科院分区：
文献类型：
--
作者：
Anderson, IC;Chambers, SM;Cairney, JWG
进行了一项实地研究，以确定澳大利亚新南威尔士州 Pisolithustinctorius 菌丝体个体的大小和空间分布。在收集和绘制果梗并将真菌分离到无菌培养物中之后，提取基因组 DNA，并结合 RAPD 和微卫星分析的数据，用于识别和绘制菌丝体个体。在现场识别出 33 个遗传上不同的个体，虽然识别出一个较大的个体（直径至少 30 m），但大多数个体看起来相对较小（< 3 m 直径）。讨论了个体大小和分布与 P.tinctorius 菌丝体在土壤中生长的关系。
A field study was conduced to determine the size and spatial distribution of mycelial individuals of Pisolithus tinctorius at a site in NSW, Australia. Following collection and mapping of carpophores and isolation of the fungi into axenic culture, genomic DNA was extracted and combined data from RAPD and microsatellite analyses used to identify and map mycelial individuals. Thirty-three genetically distinct individuals were recognized at the field site and, while one large individual (at least 30 m diam.) was identified, most individuals appeared relatively small (< 3 m diam.). The size and distribution of individuals is discussed in relation to growth of P. tinctorius mycelia through soil.