A holobiont view of island biogeography: Unravelling patterns driving the nascent diversification of a Hawaiian spider and its microbial associates
A holobiont view of island biogeography: Unravelling patterns driving the nascent diversification of a Hawaiian spider and its microbial associates
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DOI：
10.1111/mec.16301
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发表时间：
2021-12-15
影响因子：
4.9
通讯作者：
Gillespie, Rosemary G.
中科院分区：
文献类型：
--
作者：
Armstrong, Ellie E.;Perez-Lamarque, Benoit;Gillespie, Rosemary G.
宿主谱系的多样化可以受到外部环境和微生物组合的影响。在这里，我们使用了一个年轻的蜘蛛谱系，沿着一系列按时间顺序排列的火山山脉分布，来研究当蜘蛛殖民到新的地方时，它们的相关微生物群落是如何变化的。利用夏威夷岛上的粘蛛Ariamnes waikula（蜘蛛目，蜘蛛科）和更古老岛屿上的外群分类群，我们测试了“全息生物”（蜘蛛宿主、细胞内共生生物和肠道微生物群落）的每个组成部分是否由于从老火山到新火山的顺序定植而显示出相关的多样性特征。为了研究这一点，我们从宿主蜘蛛的微生物群中生成了ddRAD数据和16S rRNA基因扩增子数据。我们预计顺序定植会导致宿主蜘蛛的（门）遗传结构和微生物群落的多样性梯度。结果表明，寄主A. waikula确实是由地理隔离构成的，这表明从较老的火山到较年轻的火山的顺序殖民。同样，不同岛屿Ariamnes物种间的内共生群落也存在显著差异，而夏威夷岛A. waikula种群间的内共生群落则较为均匀。相反，我们怀疑肠道微生物群通常是环境衍生的，在所有种群和物种中都是保守的。我们的研究结果表明，全息生物的不同组成部分以不同的方式响应火山群岛的动态环境。这突出了理解全息生物不同组成部分之间相互作用的必要性，以适当地描述其演化。
The diversification of a host lineage can be influenced by both the external environment and its assemblage of microbes. Here, we use a young lineage of spiders, distributed along a chronologically arranged series of volcanic mountains, to investigate how their associated microbial communities have changed as the spiders colonized new locations. Using the stick spider Ariamnes waikula (Araneae, Theridiidae) on the island of HawaiModified Letter Turned Commai, and outgroup taxa on older islands, we tested whether each component of the "holobiont" (spider hosts, intracellular endosymbionts and gut microbial communities) showed correlated signatures of diversity due to sequential colonization from older to younger volcanoes. To investigate this, we generated ddRAD data for the host spiders and 16S rRNA gene amplicon data from their microbiota. We expected sequential colonizations to result in a (phylo)genetic structuring of the host spiders and in a diversity gradient in microbial communities. The results showed that the host A. waikula is indeed structured by geographical isolation, suggesting sequential colonization from older to younger volcanoes. Similarly, the endosymbiont communities were markedly different between Ariamnes species on different islands, but more homogeneous among A. waikula populations on the island of HawaiModified Letter Turned Commai. Conversely, the gut microbiota, which we suspect is generally environmentally derived, was largely conserved across all populations and species. Our results show that different components of the holobiont respond in distinct ways to the dynamic environment of the volcanic archipelago. This highlights the necessity of understanding the interplay between different components of the holobiont, to properly characterize its evolution.