Lipovitellins and phosvitins of the fertilized eggs during embryo growth in the oviparous lizard Podarcis sicula
Lipovitellins and phosvitins of the fertilized eggs during embryo growth in the oviparous lizard Podarcis sicula
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卵生蜥蜴 Podarcis sicula 胚胎生长过程中受精卵的脂质卵黄蛋白和卵黄高磷蛋白
DOI：
10.1002/mrd.90018
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发表时间：
2002
影响因子：
2.5
通讯作者：
E. Limatola
中科院分区：
文献类型：
--
作者：
M. Romanò;Patrizia Rosanova;Chiara Anteo;E. Limatola
在蜥蜴Podarcis sicula中，主要的卵黄原蛋白（VTG）衍生的卵黄蛋白，脂卵黄蛋白和卵黄蛋白，在接近孵化的44天的不同孵育期从产卵和受精卵的卵黄球中提取。此时，胚胎发育已经接近尾声。用饱和硫酸铵沉淀法分离卵黄蛋白，SDS-PAGE电泳分离卵黄蛋白，用抗VTG多克隆抗体进行Western blotting检测。两个110和116 kDa的卵黄脂蛋白总是存在于1，10，18和44天后产卵的鸡蛋的蛋黄。这两种蛋白质均被糖基化，并被抗/VTG抗体识别;分析了其N末端序列。在刚产下的鸡蛋中检测到四种维生素，但它们的数量在孵育过程中减少，44天后，只存在一种分子量为6.5 kDa的蛋白质。结果表明，在这种蜥蜴，胚胎发育过程中，卵黄脂蛋白保持不变，而磷酸化的蛋黄成分进行持续降解。摩尔Reprod. Dev. 63：341-348，2002.© 2002 Wiley利斯公司
In the lizard Podarcis sicula, the major vitellogenin (VTG)‐derived yolk proteins, lipovitellins and phosvitins, were extracted from the yolk globules of laid and fertilized eggs at different periods of incubation up to 44 days close to hatching. Embryonic development was almost over at this time. Yolk proteins were isolated by precipitation in saturated (NH4)2SO4, separated on SDS–PAGE and detected by Western blotting with homologous polyclonal anti/VTG antibody. Two lipovitellins of 110 and 116 kDa were always present in the yolk of laid eggs after 1, 10, 18, and 44 days from oviposition. Both these proteins were glycosylated and were recognized by the anti/VTG antibody; their N‐terminal sequences were analyzed. Four phosvitins were detected in freshly laid eggs, but their number decreased during incubation, and after 44 days only a single protein of ≅ 6.5 kDa was present. The results indicated that, in this lizard, during embryonic development, lipovitellins remain unchanged, whereas the phosphorylated components of yolk undergo continuous degradation. Mol. Reprod. Dev. 63: 341–348, 2002. © 2002 Wiley‐Liss, Inc.
Anolis pulchellus 卵黄蛋白原多肽的合成和卵黄蛋白的沉积。
DOI：
10.1016/0305-0491(95)02112-4
发表时间：
1996
期刊：
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
影响因子：
--
作者：
Morales,MH;Baerga-Santini,C;Cordero-López,N
通讯作者：
Cordero-López,N