Antimicrobial Resistance in Escherichia coli Isolates from Swine and Wild Small Mammals in the Proximity of Swine Farms and in Natural Environments in Ontario, Canada
Antimicrobial Resistance in Escherichia coli Isolates from Swine and Wild Small Mammals in the Proximity of Swine Farms and in Natural Environments in Ontario, Canada
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DOI：
10.1128/aem.01821-08
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发表时间：
2009-02-01
影响因子：
4.4
通讯作者：
Jardine, Claire
中科院分区：
文献类型：
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作者：
Kozak, Gosia K.;Boerlin, Patrick;Jardine, Claire
通常不接触抗菌剂的野生动物可通过与人类和家畜接触以及通过环境获得抗菌剂耐药性细菌。在这项研究中，我们评估的频率抗菌药物耐药性的通用大肠杆菌菌株从野生小哺乳动物（小鼠，田鼠，和鼩）和他们的栖息地（农场或自然区域）的抗菌药物耐药性的影响。此外，我们还比较了大肠埃希菌耐药的类型和频率。大肠杆菌分离物来自收集野生小型哺乳动物的同一农场的猪。农场附近的动物携带E.大肠杆菌分离株与四环素耐药决定簇比生活在自然区域的动物;对四环素的耐药性也是最常见的耐药分离株从猪（83%）。我们的结果表明，E.从生活在农场的野生小哺乳动物分离的大肠杆菌比大肠杆菌具有更高的耐药率和更频繁的多重耐药。大肠杆菌分离株来自受人类和农业活动影响较小的环境，例如自然区域。从任何野生小型哺乳动物粪便中均未回收到沙门氏菌分离株。这项研究表明，靠近食用动物农业会增加大肠杆菌感染的可能性。野生动物大肠杆菌分离株对某些抗菌药物具有耐药性，可能是由于暴露于耐药性大肠杆菌。从家畜分离的大肠杆菌菌株，这些菌株的抗性基因，或通过与动物饲料接触的抗菌剂。
Wild animals not normally exposed to antimicrobial agents can acquire antimicrobial agent-resistant bacteria through contact with humans and domestic animals and through the environment. In this study we assessed the frequency of antimicrobial resistance in generic Escherichia coli isolates from wild small mammals (mice, voles, and shrews) and the effect of their habitat (farm or natural area) on antimicrobial resistance. Additionally, we compared the types and frequency of antimicrobial resistance in E. coli isolates from swine on the same farms from which wild small mammals were collected. Animals residing in the vicinity of farms were five times more likely to carry E. coli isolates with tetracycline resistance determinants than animals living in natural areas; resistance to tetracycline was also the most frequently observed resistance in isolates recovered from swine (83%). Our results suggest that E. coli isolates from wild small mammals living on farms have higher rates of resistance and are more frequently multiresistant than E. coli isolates from environments, such as natural areas, that are less impacted by human and agricultural activities. No Salmonella isolates were recovered from any of the wild small mammal feces. This study suggests that close proximity to food animal agriculture increases the likelihood that E. coli isolates from wild animals are resistant to some antimicrobials, possibly due to exposure to resistant E. coli isolates from livestock, to the resistance genes of these isolates, or to antimicrobials through contact with animal feed.