ROLE OF THE HOST-CELL IN PERSISTENT VIRAL-INFECTION - COEVOLUTION OF L-CELLS AND REOVIRUS DURING PERSISTENT INFECTION
ROLE OF THE HOST-CELL IN PERSISTENT VIRAL-INFECTION - COEVOLUTION OF L-CELLS AND REOVIRUS DURING PERSISTENT INFECTION
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DOI：
10.1016/0092-8674(81)90050-7
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
FIELDS, BN
中科院分区：
文献类型：
--
作者：
AHMED, R;CANNING, WM;FIELDS, BN
在用抗呼肠孤病毒血清治愈持续感染的细胞系（L/C）后，分离出突变L [小鼠成纤维细胞]细胞，称为LR细胞。LR细胞是无病毒的;通过感染中心测定、空斑测定、病毒蛋白的存在、病毒ds（双链）RNA的存在和免疫荧光研究未检测到呼肠孤病毒。在用克隆的低传代野生型呼肠孤病毒或从载体培养物分离的克隆的低传代呼肠孤病毒感染后，在LR细胞中容易建立持续感染。从载体培养物中分离的呼肠孤病毒在LR细胞中比野生型呼肠孤病毒生长得好得多，并且在共感染实验中显示出对野生型呼肠孤病毒的完全优势。用野生型呼肠孤病毒感染LR细胞导致低水平的持续感染，病毒复制效率低下;这些突变L细胞对野生型呼肠孤病毒感染具有部分抗性。用从持续感染细胞中分离的病毒感染突变体L细胞导致持续感染，伴随着有效的病毒复制。用野生型呼肠孤病毒或从持续感染的细胞分离的呼肠孤病毒感染原始L细胞导致裂解性感染，没有存活的细胞。因此，宿主细胞在维持持续性呼肠孤病毒感染中起着至关重要的作用。在持续感染过程中，突变型L细胞和突变型呼肠孤病毒存在协同进化。
Mutant L [mouse fibroblast] cells, designated LR cells, were isolated after curing a persistently infected cell line (L/C) with antireovirus serum. The LR cells were virus-free; no reovirus was detectable by infectious center assays, plaque assays, presence of viral proteins, presence of viral ds(double-stranded)RNA and immunofluorescence studies. Persistent infections were readily established in LR cells following infection with either cloned, low passage wild-type reovirus or cloned, low passage reovirus isolated from carrier cultures. Reovirus isolated from carrier cultures grew much better than wild-type reovirus in LR cells and showed complete dominance over wild-type reovirus in coinfection experiments. Infection of LR cells with wild-type reovirus resulted in a low-level persistent infection with inefficient viral replication; these mutant L cells were partially resistant to infection with wild-type reovirus. Infection of the mutant L cells with virus isolated from the persistently infected cells resulted in a persistent infection accompanied with efficient viral replication. Infection of the original L cells with either wild-type reovirus or reovirus isolated from the persistently infected cells resulted in a lytic infection with no surviving cells. Thus the host cell plays a crucial role in the maintenance of persistent reovirus infection. There is apparently a coevolution of mutant L cells and mutant reovirus during persistent infection.