Isolation and Characterization of Mouse Fibroblasts
Isolation and Characterization of Mouse Fibroblasts
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DOI：
10.1007/978-1-4939-8570-8_5
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发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Redente, Elizabeth F.
中科院分区：
文献类型：
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作者：
Edelman, Benjamin L.;Redente, Elizabeth F.
肺实质由许多细胞组成，包括结构上重要的间质成纤维细胞。成纤维细胞的功能是产生细胞外基质，在肺泡上皮细胞的维持中起重要作用。为了了解成纤维细胞在体内平衡和疾病中的作用，我们分离成纤维细胞并在培养基中培养。本文介绍了两种分离和维持小鼠原代肺成纤维细胞的方法。
The lung parenchyma is comprised of many cells including the structurally important stromal fibroblasts. Fibroblasts function to produce extracellular matrix and are important in the maintenance of alveolar epithelial cells. To understand the role of fibroblasts both in homeostasis and disease, we isolate fibroblasts and grow them in culture. Two methods are presented here for the isolation and maintenance of mouse primary lung fibroblasts.