Location and distribution of Fos protein expression in rat hippocampus following acute moderate aerobic exercise
Location and distribution of Fos protein expression in rat hippocampus following acute moderate aerobic exercise
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DOI：
10.1007/s002210000634
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发表时间：
2001-03-01
影响因子：
2
通讯作者：
Waters, RS
中科院分区：
文献类型：
--
作者：
Oladehin, A;Waters, RS
海马神经元在耐力运动过程中被激活，但对这些神经元的位置和空间分布的研究较少。因此，我们使用Fos蛋白表达来确定在急性中等有氧运动中激活的海马神经元的位置和分布。成年大鼠分为训练组、训练组、非训练组和笼养组。TR组和TNR组大鼠在跑步机上跑步，每次跑20分钟，休息3分钟。UNR组的大鼠在未激活的跑步机上花费相同的时间，而CB组的大鼠在训练和实验结束后一直呆在家里的笼子里，以达到TR和TNR组的表现标准，两组都休息1天。然后，TNR组和UNR组的大鼠在跑步机上跑到标准水平，而TNR和UNR组的大鼠在未激活的跑步机上花费相同的时间。然后，所有组的动物都被处死，取出他们的大脑，切片，进行Fos蛋白免疫细胞化学处理。在海马齿状回和CA1-3区计数FOS样免疫反应(FLI)神经元。比较各组大鼠海马区FLI神经元总数及各区神经元数量。与其他三组相比，TR组海马区以及个别区域的FLI神经元总数显著增加。同样，TNR组与UNR组和CB组相比也有显著差异。相反，UNR和CB仅在CA1区存在显著差异，这可能是两组之间在海马FLI神经元总数上存在显著差异的原因。这些结果表明，在适度的体育运动中，Fos的诱导发生在海马区。此外，还讨论了加入适当的对照的重要性，以说明由于受过训练的表演、受过训练的不良表现和未受训练的群体而导致的即刻早期基因表达的可能差异。结果表明，当c-fos等即刻早期基因的表达作为结果指标时，对非运动刺激的适当控制对于研究运动对大脑的影响是必要的。
Hippocampal neurons are activated during endurance exercise; however, little attention has been given to the location and spatial distribution of these neurons. We have, therefore, used Fos protein expression to identify the location and distribution of hippocampal neurons that become activated during acute moderate aerobic exercise. Adult rats were assigned into trained running (TR), trained nonrunning (TNR), untrained nonrunning (UNR), and cage-bound (CB) groups. Rats in the TR and TNR groups were trained to run, for three 20-min running periods separated by 3 min rest, on a treadmill. Rats in the UNR group spent identical time on a nonactivated treadmill, while rats in the CB group remained in their home cages throughout the training and experimentation After training to criterion performance for both TR and TNR groups, both groups were rested for 1 day. Rats in the TR were then run on the treadmill to criterion level, while those in TNR and UNR groups spent equivalent time on the nonactivated treadmill. Animals in all groups were then killed and their brains removed, sectioned, and processed for Fos protein immunocytochemistry. Fos-like immunoreactive (FLI) neurons were counted in the dentate and CA1-3 fields of the hippocampus. The total numbers of hippocampal FLI neurons, as well as FLI neurons in each hippocampal region, were compared among groups. The total numbers of FLI neurons in the hippocampus, as well as in individual regions, were sig significantly greater in the TR group compared with the other three groups. Similarly, significant differences were found between the TNR group when compared with UNR and CB groups. Conversely, a significant difference existed between UNR and CB only in the CA1 field, which may account for the significant difference in the total number of hippocampal FLI neurons between these two groups. These results show that Fos induction occurs in the hippocampus during moderate physical exercise. Furthermore, the importance of the incorporation of adequate controls to account for possible differences in expression of immediate early gene expression due to trained performing, trained nonperforming and untrained groups is discussed. The results indicate that adequate control for nonexercise stimuli is necessary for studies of the effect of exercise on the brain when expression of immediate early genes such as c-fos is used as an outcome measure.